Open Access

Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT

  • Authors:
    • Quanchi Chen
    • Wei Sun
    • Yuxin Liao
    • Hui Zeng
    • Liancheng Shan
    • Fei Yin
    • Zhuoying Wang
    • Zifei Zhou
    • Yingqi Hua
    • Zhengdong Cai
  • View Affiliations

  • Published online on: February 18, 2015     https://doi.org/10.3892/mmr.2015.3375
  • Pages: 219-225
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Monocyte chemotactic protein‑1 (MCP‑1/CCL2) is an important immune factor, which may be important in cancer progression by promoting proliferation, invasion, metastasis and the tumor microenvironment. Previous studies have demonstrated that CCL2 affects the proliferation of osteosarcoma cells via the RANKL signaling pathway. However, the underlying mechanisms remain to be elucidated. To investigate the role of CCL2 in osteosarcoma cells, MTT, spheroid forming, wound healing and transwell assays were performed to examine the proliferation and invasion abilities of the osteosarcoma cells. It was revealed that the high‑grade osteosarcoma cells exhibited increased expression levels of CCL2 compared with the low‑grade osteosarcoma cells (P<0.001). Furthermore, knockdown of CCL2 decreased the proliferation and invasion abilities of the osteosarcoma cells (P<0.01). These results suggested that the expression of CCL2 is high in high‑grade osteosarcoma cells and promotes the proliferation and invasion of osteosarcoma cells.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Chen Q, Sun W, Liao Y, Zeng H, Shan L, Yin F, Wang Z, Zhou Z, Hua Y, Cai Z, Cai Z, et al: Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT. Mol Med Rep 12: 219-225, 2015
APA
Chen, Q., Sun, W., Liao, Y., Zeng, H., Shan, L., Yin, F. ... Cai, Z. (2015). Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT. Molecular Medicine Reports, 12, 219-225. https://doi.org/10.3892/mmr.2015.3375
MLA
Chen, Q., Sun, W., Liao, Y., Zeng, H., Shan, L., Yin, F., Wang, Z., Zhou, Z., Hua, Y., Cai, Z."Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT". Molecular Medicine Reports 12.1 (2015): 219-225.
Chicago
Chen, Q., Sun, W., Liao, Y., Zeng, H., Shan, L., Yin, F., Wang, Z., Zhou, Z., Hua, Y., Cai, Z."Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT". Molecular Medicine Reports 12, no. 1 (2015): 219-225. https://doi.org/10.3892/mmr.2015.3375