Vasoactive intestinal peptide induces CD14+HLA-DR‑/low myeloid-derived suppressor cells in gastric cancer

  • Authors:
    • Gang Li
    • Ke Wu
    • Kaixiong Tao
    • Xiaoming Lu
    • Jianhua Ma
    • Zhengqiang Mao
    • Hang Li
    • Liang Shi
    • Jing Li
    • Yanfeng Niu
    • Fan Xiang
    • Guobin Wang
  • View Affiliations

  • Published online on: February 18, 2015     https://doi.org/10.3892/mmr.2015.3374
  • Pages: 760-768
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Myeloid‑derived suppressor cells (MDSCs) are a heterogeneous group of cells, which have been revealed to inhibit T‑cell responses in tumor‑bearing mice. In addition, a number of immune suppressive mechanisms have linked MDSCs and the development of human cancer. However, the role of MDSCs in human gastric cancer tissue remains to be elucidated as specific markers are lacking. Therefore, the aim of the present study was to investigate the frequency and immune suppressive function of MDSCs denoted in the present study as cluster of differentiation 14 (CD14)+human leukocyte antigen (HLA)‑DR‑/low in gastric cancer patients. In the present study, MDSCs were directly isolated and characterized from the tumor and adjacent normal tissue of gastric cancer patients. Functional analysis of the CD14+HLA‑DR‑/low MDSCs co‑cultured with allogeneic CD4+ T cells were performed and compared with controls. In addition, the interferon‑γ (IFN‑γ) and interleukin (IL)‑2 production was compared in order to investigate the capacity of vasoactive intestinal peptide (VIP) to induce CD14+HLA‑DR‑/low MDSC‑mediated CD4+ T‑cell dysfunction and whether IL‑10 secretion is involved in this mechanism. As a result, the quantity of CD14+HLA‑DR‑/low cells in tumor tissue from gastric cancer patients was significantly higher than that in the adjacent normal tissue. In addition, CD14+HLA‑DR‑/low MDSCs isolated from tumor tissue were observed to inhibit the CD4+ T‑cells' immune responses in comparison with those from the adjacent normal tissue. Furthermore, VIP was able to induce the differentiation of CD14+ mononuclear cells isolated from healthy donor peripheral blood mononuclear cells into activated MDSC cells. Of note, the immunosuppressive effect of VIP‑induced CD14+HLA‑DR‑/low MDSCs on CD4+ T cells was mediated by IL‑10 secretion, which was demonstrated in the subsequent decrease of IFN‑γ and IL‑2 production. In conclusion, CD14+HLA‑DR‑/low cells were significantly increased in gastric cancer tissue and were shown to have a critical role in CD4+T‑cell immunosuppression. In addition, VIP as a novel cytokine may induce the differentiation of CD14+ mononuclear cells towards CD14+HLA‑DR‑/low MDSCs. An improved understanding of phenotypic heterogeneity and the mechanism of generation of MDSCs in gastric cancer patients is important in the design of effective immunotherapeutic strategies.
View Figures
View References

Related Articles

Journal Cover

July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li G, Wu K, Tao K, Lu X, Ma J, Mao Z, Li H, Shi L, Li J, Niu Y, Niu Y, et al: Vasoactive intestinal peptide induces CD14+HLA-DR‑/low myeloid-derived suppressor cells in gastric cancer. Mol Med Rep 12: 760-768, 2015
APA
Li, G., Wu, K., Tao, K., Lu, X., Ma, J., Mao, Z. ... Wang, G. (2015). Vasoactive intestinal peptide induces CD14+HLA-DR‑/low myeloid-derived suppressor cells in gastric cancer. Molecular Medicine Reports, 12, 760-768. https://doi.org/10.3892/mmr.2015.3374
MLA
Li, G., Wu, K., Tao, K., Lu, X., Ma, J., Mao, Z., Li, H., Shi, L., Li, J., Niu, Y., Xiang, F., Wang, G."Vasoactive intestinal peptide induces CD14+HLA-DR‑/low myeloid-derived suppressor cells in gastric cancer". Molecular Medicine Reports 12.1 (2015): 760-768.
Chicago
Li, G., Wu, K., Tao, K., Lu, X., Ma, J., Mao, Z., Li, H., Shi, L., Li, J., Niu, Y., Xiang, F., Wang, G."Vasoactive intestinal peptide induces CD14+HLA-DR‑/low myeloid-derived suppressor cells in gastric cancer". Molecular Medicine Reports 12, no. 1 (2015): 760-768. https://doi.org/10.3892/mmr.2015.3374