Open Access

Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats

  • Authors:
    • Ying‑Qin Li
    • Ying Tang
    • Rao Fu
    • Qiu‑Hua Meng
    • Xue Zhou
    • Ze‑Min Ling
    • Xiao Cheng
    • Su‑Wei Tian
    • Guo‑Jie Wang
    • Xue‑Guo Liu
    • Li‑Hua Zhou
  • View Affiliations

  • Published online on: March 23, 2015     https://doi.org/10.3892/mmr.2015.3532
  • Pages: 913-920
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Although studies have been undertaken on gadolinium labeling‑based molecular imaging in magnetic resonance imaging (MRI), the use of non‑ionic gadolinium in the tracking of stem cells remains uncommon. To investigate the efficiency in tracking of stem cells with non‑ionic gadolinium as an MRI contrast agent, a rhodamine‑conjugated fluorescent reagent was used to label bone marrow stromal cells (BMSCs) of neonatal rats in vitro, and MRI scanning was undertaken. The fluorescent‑conjugated cell uptake reagents were able to deliver gadodiamide into BMSCs, and cell uptake was verified using flow cytometry. In addition, the labeled stem cells with paramagnetic contrast medium remained detectable by an MRI monitor for a minimum of 28 days. The present study suggested that this method can be applied efficiently and safely for the labeling and tracking of bone marrow stromal cells in neonatal rats.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Li YQ, Tang Y, Fu R, Meng QH, Zhou X, Ling ZM, Cheng X, Tian SW, Wang GJ, Liu XG, Liu XG, et al: Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats. Mol Med Rep 12: 913-920, 2015
APA
Li, Y., Tang, Y., Fu, R., Meng, Q., Zhou, X., Ling, Z. ... Zhou, L. (2015). Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats. Molecular Medicine Reports, 12, 913-920. https://doi.org/10.3892/mmr.2015.3532
MLA
Li, Y., Tang, Y., Fu, R., Meng, Q., Zhou, X., Ling, Z., Cheng, X., Tian, S., Wang, G., Liu, X., Zhou, L."Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats". Molecular Medicine Reports 12.1 (2015): 913-920.
Chicago
Li, Y., Tang, Y., Fu, R., Meng, Q., Zhou, X., Ling, Z., Cheng, X., Tian, S., Wang, G., Liu, X., Zhou, L."Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats". Molecular Medicine Reports 12, no. 1 (2015): 913-920. https://doi.org/10.3892/mmr.2015.3532