Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats

  • Authors:
    • Jin‑Shan Sun
    • Yu‑Jie Yang
    • Yong‑Zhen Zhang
    • Wen Huang
    • Zhao‑Shen Li
    • Yong Zhang
  • View Affiliations

  • Published online on: May 6, 2015     https://doi.org/10.3892/mmr.2015.3735
  • Pages: 2677-2682
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Abstract

The mechanisms associated with diabetes‑induced neuropathic pain are complex and poorly understood. In order to understand the involvement of spinal microglia activity in diabetic pain, the present study investigated whether minocycline treatment is able to attenuate diabetic pain using a rat model. Diabetes was induced using a single intraperitoneal injection of streptozotocin (STZ). Minocycline was then intrathecally administered to the rats. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested weekly. The expression of OX‑42, Iba‑1, phospho‑p38 mitogen‑activated protein kinase (MAPK), tumor necrosis factor‑α (TNF‑α), interleukin‑1β (IL‑1β) and inducible nitric oxide synthase (iNOS), were examined in the spinal cord in order to evaluate the activation of microglia. The present study demonstrated that rats with STZ‑induced diabetes exhibited increased mean plasma glucose concentration, decreased mean body weight and significant pain hypersensitivity compared with control rats. PWT and PWL values of rats with STZ‑induced diabetes increased following treatment with minocycline. No differences were observed in expression levels of the microglial activity markers (OX‑42, Iba‑1 and phospho‑p38 MAPK) between rats with STZ‑induced diabetes and control rats. However, TNF‑α, IL‑1β and iNOS expression levels were higher in rats with STZ‑induced diabetes compared with control rats. Following treatment with minocycline markers of microglial activation, including cytokines and iNOS, were downregulated in rats with STZ‑induced diabetes. The results of the present study indicated that minocycline treatment may inhibit spinal microglial activation and attenuate diabetic pain in rats with STZ‑induced diabetes.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun JS, Yang YJ, Zhang YZ, Huang W, Li ZS and Zhang Y: Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats. Mol Med Rep 12: 2677-2682, 2015
APA
Sun, J., Yang, Y., Zhang, Y., Huang, W., Li, Z., & Zhang, Y. (2015). Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats. Molecular Medicine Reports, 12, 2677-2682. https://doi.org/10.3892/mmr.2015.3735
MLA
Sun, J., Yang, Y., Zhang, Y., Huang, W., Li, Z., Zhang, Y."Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats". Molecular Medicine Reports 12.2 (2015): 2677-2682.
Chicago
Sun, J., Yang, Y., Zhang, Y., Huang, W., Li, Z., Zhang, Y."Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats". Molecular Medicine Reports 12, no. 2 (2015): 2677-2682. https://doi.org/10.3892/mmr.2015.3735