Suppression of microRNA-18a expression inhibits invasion and promotes apoptosis of human trophoblast cells by targeting the estrogen receptor α gene

  • Authors:
    • Xiaoming Zhu
    • Yang Yang
    • Tao Han
    • Guowu Yin
    • Ping Gao
    • Yunfeng Ni
    • Xiaohua Su
    • Yuying Liu
    • Yuanqing Yao
  • View Affiliations

  • Published online on: May 4, 2015     https://doi.org/10.3892/mmr.2015.3724
  • Pages: 2701-2706
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Abstract

The purpose of the present study was to gain further understanding of the function of microRNA-18a (miR-18a) expression in the JEG‑3 human trophoblast cell line. JEG‑3 cells were transfected with pre‑miR‑18a mimics or miR‑18a inhibitors. The effects of miR‑18a on trophoblast cell invasion and apoptosis, and on the expression of estrogen receptor α (ESRα) were analyzed using a Transwell invasion assay, flow cytometry, reverse transcription‑polymerase chain reaction, western blot analysis and a luciferase assay. The results of the present study suggested that miR‑18a expression suppression led to a decrease in JEG‑3 cell invasion and an increase in JEG‑3 cell apoptosis, by inducing ESRα expression. The present study provides evidence for the involvement of miR‑18a in the pathogenesis of pre-eclamptic pregnancies.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu X, Yang Y, Han T, Yin G, Gao P, Ni Y, Su X, Liu Y and Yao Y: Suppression of microRNA-18a expression inhibits invasion and promotes apoptosis of human trophoblast cells by targeting the estrogen receptor α gene. Mol Med Rep 12: 2701-2706, 2015
APA
Zhu, X., Yang, Y., Han, T., Yin, G., Gao, P., Ni, Y. ... Yao, Y. (2015). Suppression of microRNA-18a expression inhibits invasion and promotes apoptosis of human trophoblast cells by targeting the estrogen receptor α gene. Molecular Medicine Reports, 12, 2701-2706. https://doi.org/10.3892/mmr.2015.3724
MLA
Zhu, X., Yang, Y., Han, T., Yin, G., Gao, P., Ni, Y., Su, X., Liu, Y., Yao, Y."Suppression of microRNA-18a expression inhibits invasion and promotes apoptosis of human trophoblast cells by targeting the estrogen receptor α gene". Molecular Medicine Reports 12.2 (2015): 2701-2706.
Chicago
Zhu, X., Yang, Y., Han, T., Yin, G., Gao, P., Ni, Y., Su, X., Liu, Y., Yao, Y."Suppression of microRNA-18a expression inhibits invasion and promotes apoptosis of human trophoblast cells by targeting the estrogen receptor α gene". Molecular Medicine Reports 12, no. 2 (2015): 2701-2706. https://doi.org/10.3892/mmr.2015.3724