Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway

  • Authors:
    • Barun Poudel
    • Sarmila Nepali
    • Mingjie Xin
    • Hyeon‑Hui Ki
    • Young‑Ho Kim
    • Dae‑Ki Kim
    • Young‑Mi Lee
  • View Affiliations

  • Published online on: April 29, 2015     https://doi.org/10.3892/mmr.2015.3700
  • Pages: 3139-3145
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Abstract

The present study aimed to compare the potential anti-adipogenic effects and underlying mechanisms of the luteolin, isoscoparin and isoorientin flavonoids, purified from Triticum aestivum sprout (TA) in 3T3‑L1 cells. The cells were treated with different concentrations of flavonoids for 8 days and the lipid accumulation was assessed using Oil‑Red‑O staining. The expression levels of the transcription factors and the genes involved in adipogenesis in the cells were assessed by reverse transcription‑quantitative polymerase chain reaction and western blotting. The results demonstrated that 10 µM luteolin, isoscoparin or isoorientin inhibited lipid deposition in the cells by 74, 63 and 65%, respectively. The flavonoids also significantly inhibited the transcriptional regulators of adipogenesis, including peroxisome proliferator‑activated receptor‑γ, CAAT/enhancer binding protein‑α and sterol regulatory element binding protein (SREBP)‑1c, compared with the control cells. Similarly, there was a significant downregulation of the adipocyte specific markers associated with lipid metabolism, including activating protein‑2, fatty acid synthase, hormone‑sensitive lipase and lipoprotein lipase, in the flavonoid treated cells. Notably, the cells treated with the flavonoids demonstrated increased expression levels of the insulin‑induced genes, insig‑1 and insig‑2, which may have inhibited the activation of the adipogenic transcription factor, SREBP, eventually leading to the inhibition of adipogenesis. Taken together, these results revealed that the flavonoids from TA possessed an inhibitory effect on adipogenesis through downregulation of adipogenic transcription factors and genes associated with lipid metabolism, and the upregulation of insig 1 and 2, suggesting that the flavonoids from TA may be potential therapeutic agents for the prevention and treatment of obesity.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Poudel B, Nepali S, Xin M, Ki HH, Kim YH, Kim DK and Lee YM: Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway. Mol Med Rep 12: 3139-3145, 2015
APA
Poudel, B., Nepali, S., Xin, M., Ki, H., Kim, Y., Kim, D., & Lee, Y. (2015). Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway. Molecular Medicine Reports, 12, 3139-3145. https://doi.org/10.3892/mmr.2015.3700
MLA
Poudel, B., Nepali, S., Xin, M., Ki, H., Kim, Y., Kim, D., Lee, Y."Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway". Molecular Medicine Reports 12.2 (2015): 3139-3145.
Chicago
Poudel, B., Nepali, S., Xin, M., Ki, H., Kim, Y., Kim, D., Lee, Y."Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway". Molecular Medicine Reports 12, no. 2 (2015): 3139-3145. https://doi.org/10.3892/mmr.2015.3700