Increase in osteoclastogenesis in an obese Otsuka Long-Evans Tokushima fatty rat model

  • Authors:
    • Tomoyo Ootsuka
    • Atsuko Nakanishi
    • Ikuyo Tsukamoto
  • View Affiliations

  • Published online on: May 21, 2015     https://doi.org/10.3892/mmr.2015.3811
  • Pages: 3874-3880
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the present study, the effects of obesity on bone metabolism were investigated using a hyperphagic and obese rat model, the Otsuka Long‑Evans Tokushima fatty (OLETF) rat, which exhibits normal glycemic control at 8 weeks of age. Body weight, food intake, fat mass, markers of bone resorption, the activities of tartrate‑resistant acid phosphatase (TRAP) and cathepsin K, the number of osteoclasts in the proximal tibia, and the serum C‑terminal crosslinking telopeptide level were higher in OLETF rats than those in control rats (Long‑Evans Tokushima Otsuka; LETO). However, no differences in markers of bone formation, alkaline phosphatase activity, the number of osteoblasts in the proximal tibia or the serum osteocalcin level were observed. mRNA and protein levels of c‑fms, receptor for activation of nuclear factor‑κB (RANK), RANK ligand (RANKL), TRAP and cathepsin K were significantly increased in OLETF rats, although those levels of macrophage colony‑stimulating factor (M‑CSF) and osteoprotegerin (OPG) were similar to those in LETO rats. The level of serum tumor necrosis factor α (TNFα), and that of TNFα mRNA in bone, increased in association with the activation of NFκB. Furthermore, a frequency analysis and a colony formation assay respectively showed that the number of osteoclast precursors and the number of colony‑forming cells induced by M‑CSF each increased in OLETF rats compared with the control group. These results suggested that hyperphagia‑induced obesity with normal glycemic control induces the upregulation of osteoclastogenesis that is associated with an increase in the expression of c‑fms, RANK and RANKL, which is induced by TNFα, via the activation of NFκB.
View Figures
View References

Related Articles

Journal Cover

September-2015
Volume 12 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ootsuka T, Nakanishi A and Tsukamoto I: Increase in osteoclastogenesis in an obese Otsuka Long-Evans Tokushima fatty rat model. Mol Med Rep 12: 3874-3880, 2015
APA
Ootsuka, T., Nakanishi, A., & Tsukamoto, I. (2015). Increase in osteoclastogenesis in an obese Otsuka Long-Evans Tokushima fatty rat model. Molecular Medicine Reports, 12, 3874-3880. https://doi.org/10.3892/mmr.2015.3811
MLA
Ootsuka, T., Nakanishi, A., Tsukamoto, I."Increase in osteoclastogenesis in an obese Otsuka Long-Evans Tokushima fatty rat model". Molecular Medicine Reports 12.3 (2015): 3874-3880.
Chicago
Ootsuka, T., Nakanishi, A., Tsukamoto, I."Increase in osteoclastogenesis in an obese Otsuka Long-Evans Tokushima fatty rat model". Molecular Medicine Reports 12, no. 3 (2015): 3874-3880. https://doi.org/10.3892/mmr.2015.3811