Open Access

Liraglutide protects pancreatic β-cells against free fatty acids in vitro and affects glucolipid metabolism in apolipoprotein E‑/‑ mice by activating autophagy

  • Authors:
    • Jia Wang
    • Jie Wu
    • Hong Wu
    • Xingzhen Liu
    • Yingjian Chen
    • Jianying Wu
    • Chengjin Hu
    • Dajin Zou
  • View Affiliations

  • Published online on: June 16, 2015     https://doi.org/10.3892/mmr.2015.3944
  • Pages: 4210-4218
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The aim of the present study was to determine whether liraglutide (LRG), a long acting glucagon-like peptide 1 analogue, exerted a protective effect on free fatty acid (FFA)‑treated pancreatic β‑cells via activating autophagy. INS‑1 insulinoma pancreatic islet cell lines were treated with FFA and the levels of cell necrosis, apoptosis and autophagy were detected using an MTT assay, flow cytometry and electron microscopy (ECM). A type 2 diabetes mellitus mouse model was established through treatment of mice with a high‑fat diet for 8 weeks and injection of streptozotocin. LRG and autophagy inhibitors were used to investigate the protective effect of LRG on pancreatic β‑cells in vivo. Metabolic indices were measured and pancreatic autophagy was detected. In the INS‑1 cells, viability was higher in the FFA + LRG group compared with the FFA group, while the apoptotic rate was lower (P<0.05). The light chain 3B and p62 autophagy‑associated proteins were upregulated by LRG, while ATG7 and Beclin1 were downregulated. Autophagy inhibitors reduced the protective effect of LRG in the FFA‑treated INS‑1 cells. The type 2 diabetes mouse model was successfully established, termed the HF group, in which LRG was observed to reduce body weight and decrease levels of fasting blood glucose, total cholesterol, serum insulin, triglyceride, low density lipoprotein‑cholesterol and glycosylated hemoglobin (P<0.05), compared with the HF group. However, chloroquine treatment abrogated these effects (P<0.05, compared with the HF + LRG group; P>0.05, compared with the HF group). Autophagosomes were also observed under ECM in the pancreatic tissues of mice in the HF + LRG group. Therefore, LRG induced autophagy and exerted protective effects on pancreatic β-cells in vitro and in vivo.
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September-2015
Volume 12 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang J, Wu J, Wu H, Liu X, Chen Y, Wu J, Hu C and Zou D: Liraglutide protects pancreatic β-cells against free fatty acids in vitro and affects glucolipid metabolism in apolipoprotein E‑/‑ mice by activating autophagy. Mol Med Rep 12: 4210-4218, 2015
APA
Wang, J., Wu, J., Wu, H., Liu, X., Chen, Y., Wu, J. ... Zou, D. (2015). Liraglutide protects pancreatic β-cells against free fatty acids in vitro and affects glucolipid metabolism in apolipoprotein E‑/‑ mice by activating autophagy. Molecular Medicine Reports, 12, 4210-4218. https://doi.org/10.3892/mmr.2015.3944
MLA
Wang, J., Wu, J., Wu, H., Liu, X., Chen, Y., Wu, J., Hu, C., Zou, D."Liraglutide protects pancreatic β-cells against free fatty acids in vitro and affects glucolipid metabolism in apolipoprotein E‑/‑ mice by activating autophagy". Molecular Medicine Reports 12.3 (2015): 4210-4218.
Chicago
Wang, J., Wu, J., Wu, H., Liu, X., Chen, Y., Wu, J., Hu, C., Zou, D."Liraglutide protects pancreatic β-cells against free fatty acids in vitro and affects glucolipid metabolism in apolipoprotein E‑/‑ mice by activating autophagy". Molecular Medicine Reports 12, no. 3 (2015): 4210-4218. https://doi.org/10.3892/mmr.2015.3944