Open Access

Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration

  • Authors:
    • Peng Hu
    • Bo Feng
    • Guanglin Wang
    • Bin Ning
    • Tanghong Jia
  • View Affiliations

  • Published online on: July 2, 2015     https://doi.org/10.3892/mmr.2015.4022
  • Pages: 4925-4930
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore the underlying mechanism of the development of intervertebral disc degeneration (IDD) by bioinformatics based on microarray datasets. GSE 19943 and GSE 34095 datasets downloaded from Gene Expression Omnibus data were used to screen the differentially expressed genes (DEGs) in IDD. The correlation between microRNAs and target genes was investigated using different algorithms. The underlying molecular mechanisms of the target genes were then explored using Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology function enrichment analysis. A total of 9 differentially expressed microRNAs, including 3 down‑ and 6 upregulated microRNAs and 850 DEGs were identified in tissue from patients with IDD. Two regulation networks of the target genes by microRNAs were constructed, including 33 upregulated microRNA‑target gene pairs and 4 downregulated microRNA‑target gene pairs. Certain target genes had been demonstrated to be involved in IDD progression via various pathways, including in the cell cycle and pathways in cancer. In addition, two important microRNAs (microRNA‑222 and microRNA‑589) were identified that were pivotal for the development of IDD, and their target genes, CDKNAB and SMAD4. In conclusion, a comprehensive miRNA‑target gene regulatory network was constructed, which was found to be important in IDD progression.
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October-2015
Volume 12 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hu P, Feng B, Wang G, Ning B and Jia T: Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration. Mol Med Rep 12: 4925-4930, 2015
APA
Hu, P., Feng, B., Wang, G., Ning, B., & Jia, T. (2015). Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration. Molecular Medicine Reports, 12, 4925-4930. https://doi.org/10.3892/mmr.2015.4022
MLA
Hu, P., Feng, B., Wang, G., Ning, B., Jia, T."Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration". Molecular Medicine Reports 12.4 (2015): 4925-4930.
Chicago
Hu, P., Feng, B., Wang, G., Ning, B., Jia, T."Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration". Molecular Medicine Reports 12, no. 4 (2015): 4925-4930. https://doi.org/10.3892/mmr.2015.4022