Open Access

Effects of rotigaptide (ZP123) on connexin43 remodeling in canine ventricular fibrillation

Corrigendum in: /mmr/12/6/8327

  • Authors:
    • Guo-Ying Su
    • Jing Wang
    • Zhen-Xing Xu
    • Xiao-Jun Qiao
    • Jing-Quan Zhong
    • Yun Zhang
  • View Affiliations

  • Published online on: August 6, 2015     https://doi.org/10.3892/mmr.2015.4193
  • Pages: 5746-5752
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the effects of rotigaptide (ZP123) on the expression, distribution and phosphorylation of connexin43 (Cx43) in myocardial cell membranes in cardioversion of ventricular fibrillation (VF). A model of prolonged VF (8, 12 and 30 min) was established in mongrel dogs (n=8/group), following treatment with ZP123 or normal saline (NS control). A sham control was included. Cardiopulmonary resuscitation was begun at the start of VF followed by defibrillation. Animals received a maximum of three defibrillations of increasing energy (70, 100 and 150 J biphasic shock) as required. The average defibrillation energy, defibrillation success rate, return of spontaneous circulation and survival rate were recorded. Cx43 and phosphorylated (p-)Cx43 expression in cardiomyocyte membranes was detected by western blot and immunofluorescence analyses. Compared with the NS-treated control groups, the success defibrillation rate in the 8-min and 12-min ZP123 groups was significantly higher (P<0.05), while the average defibrillation energy was significantly lower (P<0.05). Cx43 expression in the VF groups was significantly lower than that in the sham control group (P<0.05). Cx43 expression was higher in the 12-min and 30-min ZP123 groups than that in the NS control group (P<0.05), while p-Cx43 expression decreased, although the levels were significantly higher than those in the control groups (P<0.05). Cx43 expression was positively correlated with the defibrillation success rate (r=0.91; P<0.01) and negatively with the mean defibrillation energy (r=-0.854; P<0.01), while p-Cx43 expression was positively correlated with the success rate of the previous three defibrillations (r=0.926; P<0.01).In conclusion, ZP123 reduced Cx43 remodeling through regulating the expression, distribution and phosphorylation of Cx43, thereby reducing the defibrillation energy required for successful cardioversion.
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October-2015
Volume 12 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Su G, Wang J, Xu Z, Qiao X, Zhong J and Zhang Y: Effects of rotigaptide (ZP123) on connexin43 remodeling in canine ventricular fibrillation Corrigendum in /mmr/12/6/8327. Mol Med Rep 12: 5746-5752, 2015
APA
Su, G., Wang, J., Xu, Z., Qiao, X., Zhong, J., & Zhang, Y. (2015). Effects of rotigaptide (ZP123) on connexin43 remodeling in canine ventricular fibrillation Corrigendum in /mmr/12/6/8327. Molecular Medicine Reports, 12, 5746-5752. https://doi.org/10.3892/mmr.2015.4193
MLA
Su, G., Wang, J., Xu, Z., Qiao, X., Zhong, J., Zhang, Y."Effects of rotigaptide (ZP123) on connexin43 remodeling in canine ventricular fibrillation Corrigendum in /mmr/12/6/8327". Molecular Medicine Reports 12.4 (2015): 5746-5752.
Chicago
Su, G., Wang, J., Xu, Z., Qiao, X., Zhong, J., Zhang, Y."Effects of rotigaptide (ZP123) on connexin43 remodeling in canine ventricular fibrillation Corrigendum in /mmr/12/6/8327". Molecular Medicine Reports 12, no. 4 (2015): 5746-5752. https://doi.org/10.3892/mmr.2015.4193