Open Access

Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro

  • Authors:
    • Wu Yao
    • Suna Liu
    • Ju Li
    • Changfu Hao
  • View Affiliations

  • Published online on: August 11, 2015     https://doi.org/10.3892/mmr.2015.4212
  • Pages: 5828-5836
  • Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To investigate the effects of silica on circulating fibrocytes (cFbs), the present study established a primary culture model of rat alveolar macrophages and cFbs in vitro. Macrophages were treated with free silica, and their supernatant was used to stimulate cFbs. The mRNA expression levels of collagen I, collagen III and α‑smooth muscle actin (SMA) in cFbs were analyzed by reverse transcription‑quantitative polymerase chain reaction. The intracellular and extracellular protein expression levels of collagen I, collagen III and α‑SMA were detected by ELISA and immunofluorescence staining. The results indicated that in the cell model, the free silica effectively increased the protein and mRNA expression levels of collagen‑I, collagen‑III and α‑SMA. The free silica significantly promoted the transdifferentiation of cFbs into myofibroblasts in a dose‑and time-dependent manner.
View Figures
View References

Related Articles

Journal Cover

October-2015
Volume 12 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yao W, Liu S, Li J and Hao C: Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro. Mol Med Rep 12: 5828-5836, 2015
APA
Yao, W., Liu, S., Li, J., & Hao, C. (2015). Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro. Molecular Medicine Reports, 12, 5828-5836. https://doi.org/10.3892/mmr.2015.4212
MLA
Yao, W., Liu, S., Li, J., Hao, C."Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro". Molecular Medicine Reports 12.4 (2015): 5828-5836.
Chicago
Yao, W., Liu, S., Li, J., Hao, C."Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro". Molecular Medicine Reports 12, no. 4 (2015): 5828-5836. https://doi.org/10.3892/mmr.2015.4212