Open Access

MicroRNA‑144 inhibits migration and proliferation in rectal cancer by downregulating ROCK‑1

  • Authors:
    • Shang‑Dang Cai
    • Jian‑She Chen
    • Zuo‑Wu Xi
    • Long‑Jiang Zhang
    • Ming‑Liao Niu
    • Zong‑Yue Gao
  • View Affiliations

  • Published online on: September 30, 2015     https://doi.org/10.3892/mmr.2015.4391
  • Pages: 7396-7402
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer of the colon and rectum are two distinct entities, which require different treatment strategies and separate treatment. MicroRNAs (miRNAs) act as critical regulators of genes involved in several biological processes. Aberrant alterations of miRNAs have been found in several types of cancer, including colon cancer and rectal cancer. Extensive catalogues of downregulated miRNAs have been identified for colon cancer, whereas only limited data are available for rectal cancer. An example of miRNA profiling in a previous study found that miRNA (miR)‑144 showed aberrant expression and appeared to be rectal cancer‑specific, its expression not being reported in colon cancer. In the present study, the role of miR‑144 in rectal cancer was investigated. SW837 and SW1463 cell lines were selected as rectal cell carcinoma cells. Using reverse transcription-quantitative polymerase chain reaction, western blot, BrdU, cell migration and cell viability assays, it was found that the expression levels of miR‑144 were significantly reduced in the SW837 and SW1463 cell lines, and the overexpression of miR‑144 suppressed rectal cancer cell viability, migration and proliferation. In addition, Rho‑associated coiled‑coil containing protein kinase 1 (ROCK1) was identified as a target of miR‑144 in the rectal cancer cells. The supplementation of ROCK1 markedly restored the cell migration and proliferation, which was inhibited by miR‑144. Together, the data of the present study demonstrated that miR‑144 acts as a tumor suppressor by targeting ROCK1, and indicates the potential of miR‑144 as a novel biomarker and target in the treatment of rectal cancer.
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November-2015
Volume 12 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cai SD, Chen JS, Xi ZW, Zhang LJ, Niu ML and Gao ZY: MicroRNA‑144 inhibits migration and proliferation in rectal cancer by downregulating ROCK‑1. Mol Med Rep 12: 7396-7402, 2015
APA
Cai, S., Chen, J., Xi, Z., Zhang, L., Niu, M., & Gao, Z. (2015). MicroRNA‑144 inhibits migration and proliferation in rectal cancer by downregulating ROCK‑1. Molecular Medicine Reports, 12, 7396-7402. https://doi.org/10.3892/mmr.2015.4391
MLA
Cai, S., Chen, J., Xi, Z., Zhang, L., Niu, M., Gao, Z."MicroRNA‑144 inhibits migration and proliferation in rectal cancer by downregulating ROCK‑1". Molecular Medicine Reports 12.5 (2015): 7396-7402.
Chicago
Cai, S., Chen, J., Xi, Z., Zhang, L., Niu, M., Gao, Z."MicroRNA‑144 inhibits migration and proliferation in rectal cancer by downregulating ROCK‑1". Molecular Medicine Reports 12, no. 5 (2015): 7396-7402. https://doi.org/10.3892/mmr.2015.4391