Additive effects of eukaryotic co‑expression plasmid carrying GRIM‑19 and LKB1 genes on breast cancer in vitro and in vivo

Retraction in: /10.3892/mmr.2021.12226

  • Authors:
    • Wei Zhang
    • Ying Shao
    • Ye Du
    • Wei Geng
    • Tong Jiang
    • Haipeng Liu
    • Duo Zhang
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  • Published online on: September 30, 2015     https://doi.org/10.3892/mmr.2015.4393
  • Pages: 7665-7672
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Abstract

Gene associated with retinoid‑interferon‑induced mortality 19 (GRIM‑19) and the liver kinase B1 (LKB1) gene, two types of tumor suppressor gene, have been demonstrated to have important roles in breast carcinogenesis. The present study developed a dual expression plasmid that co‑expressed GRIM‑19 and LKB1, and evaluated the combined effects of the two genes against breast cancer in vitro and in vivo. Transfection with a plasmid for the simultaneous expression of GRIM‑19 and LKB1 (pGRIM19‑LKB1) into MCF‑7 breast cancer cells significantly inhibited the proliferation, colony formation, migration and invasion compared with the effects of transfection with either pGRIM‑19 or pLKB1 alone. Furthermore, transfection with pGRIM19‑LKB1 induced enhanced levels of apoptosis and cell cycle arrest at G0/G1 stage in MCF7 cells compared to the effects of pGRIM‑19 or pLKB1 alone. An in vivo experiment using an MCF‑7 xenograft tumor model demonstrated that intravenous injection of pGRIM19‑LKB1 had an enhanced effect on tumor growth inhibition compared to that of pGRIM‑19 or pLKB1 alone. In conclusion the findings of the present study suggested that transfection with eukaryotic plasmid for the simultaneous expression of GRIM‑19 and LKB1 more effectively suppressed the growth of breast cancer in vitro and in vivo, and may therefore have therapeutic potential for the treatment of human breast cancer.
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November-2015
Volume 12 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang W, Shao Y, Du Y, Geng W, Jiang T, Liu H and Zhang D: Additive effects of eukaryotic co‑expression plasmid carrying GRIM‑19 and LKB1 genes on breast cancer in vitro and in vivo Retraction in /10.3892/mmr.2021.12226. Mol Med Rep 12: 7665-7672, 2015
APA
Zhang, W., Shao, Y., Du, Y., Geng, W., Jiang, T., Liu, H., & Zhang, D. (2015). Additive effects of eukaryotic co‑expression plasmid carrying GRIM‑19 and LKB1 genes on breast cancer in vitro and in vivo Retraction in /10.3892/mmr.2021.12226. Molecular Medicine Reports, 12, 7665-7672. https://doi.org/10.3892/mmr.2015.4393
MLA
Zhang, W., Shao, Y., Du, Y., Geng, W., Jiang, T., Liu, H., Zhang, D."Additive effects of eukaryotic co‑expression plasmid carrying GRIM‑19 and LKB1 genes on breast cancer in vitro and in vivo Retraction in /10.3892/mmr.2021.12226". Molecular Medicine Reports 12.5 (2015): 7665-7672.
Chicago
Zhang, W., Shao, Y., Du, Y., Geng, W., Jiang, T., Liu, H., Zhang, D."Additive effects of eukaryotic co‑expression plasmid carrying GRIM‑19 and LKB1 genes on breast cancer in vitro and in vivo Retraction in /10.3892/mmr.2021.12226". Molecular Medicine Reports 12, no. 5 (2015): 7665-7672. https://doi.org/10.3892/mmr.2015.4393