Andrographolide inhibits multiple myeloma cells by inhibiting the TLR4/NF-κB signaling pathway

  • Authors:
    • Hui Gao
    • Jianrong Wang
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  • Published online on: December 22, 2015     https://doi.org/10.3892/mmr.2015.4703
  • Pages: 1827-1832
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Abstract

Andrographolide is an active component from the extract of Andrographis paniculata [(Burm.f) Nees], a medicinal plant from the Acanthaceae family. Pharmacological studies have revealed that andrographolide possesses anti-bacterial, anti-inflammatory, anti-viral, immune regulatory and hepatoprotective properties, and is efficacious in the treatment of cardiovascular diseases, while exhibiting low toxicity and low cost. The present study aimed to determine the inhibitory effects of andrographolide on the growth of multiple myeloma (MM) cells and its possible impact on the Toll-like receptor (TLR)4/nuclear factor (NF)‑κB signaling pathway. Cell proliferation was detected using an MTT assay, cellular apoptosis was measured using flow cytometry, and caspase‑9/3 activation were assessed using colorimetric assay kits. Furthermore, TLR4 and NF‑κB protein expression was determined by western blot analysis. The results revealed that andrographolide reduced the proliferation, while increasing cellular apoptosis and caspase‑9/3 activation of MM cells, in addition to downregulating the expression of TLR4 and NF‑κB protein. Of note, TLR4- or NF-κB-targeting small-interfering (si)RNA enhanced the andrographolide‑induced inhibition of cell proliferation and induction of apoptosis of MM cells. The results of the present study therefore suggested that andrographolide inhibited multiple myeloma cells via the TLR4/NF‑κB signaling pathway.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Gao H and Gao H: Andrographolide inhibits multiple myeloma cells by inhibiting the TLR4/NF-κB signaling pathway. Mol Med Rep 13: 1827-1832, 2016
APA
Gao, H., & Gao, H. (2016). Andrographolide inhibits multiple myeloma cells by inhibiting the TLR4/NF-κB signaling pathway. Molecular Medicine Reports, 13, 1827-1832. https://doi.org/10.3892/mmr.2015.4703
MLA
Gao, H., Wang, J."Andrographolide inhibits multiple myeloma cells by inhibiting the TLR4/NF-κB signaling pathway". Molecular Medicine Reports 13.2 (2016): 1827-1832.
Chicago
Gao, H., Wang, J."Andrographolide inhibits multiple myeloma cells by inhibiting the TLR4/NF-κB signaling pathway". Molecular Medicine Reports 13, no. 2 (2016): 1827-1832. https://doi.org/10.3892/mmr.2015.4703