Survivin activates NF‑κB p65 via the IKKβ promoter in esophageal squamous cell carcinoma

  • Authors:
    • Wei Zeng
    • Hui Li
    • Yan Chen
    • Hongbo Lv
    • Ling Liu
    • Jihua Ran
    • Xiaohong Sun
    • Shayahati Bieerkehazhi
    • Yining Liu
    • Xiaomiao Li
    • Wenting Lai
    • Jibieke Watibieke
    • Meiliwuerti Dawulietihan
    • Xiumei Li
    • Huiwu Li
  • View Affiliations

  • Published online on: December 30, 2015     https://doi.org/10.3892/mmr.2015.4737
  • Pages: 1869-1880
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Abstract

Survivin and transcription factor p65 (NF‑κB p65) participate in the progression of esophageal squamous cell carcinoma (ESCC). However, the mechanism of NF‑κB p65 activation in ESCC remains to be elucidated. The aim of the present study was to investigate the role of survivin in the activation of NF‑κB p65 in ESCC. The expression levels of survivin, NF‑κB p65, inhibitor of nuclear factor κB kinase subunit α (IKKα) and inhibitor of nuclear factor κB kinase subunit β (IKKβ) were detected in ESCC tissue samples. Eca109 and KYSE150 cells were cultured and survivin activity was modulated via transfection with an overexpression plasmid, a small hairpin RNA plasmid and a specific inhibitor. Quantitative reverse transcription‑polymerase chain reaction and western blotting assays were conducted to assess the effects of survivin on the expression levels of IKKα, IKKβ and NF‑κB p65. Cell cycle and apoptosis assays were conducted to detect survivin‑dependent cellular behavior changes. In addition, the luciferase reporter gene assay and chromatin immunoprecipitation assay were conducted to determine the genomic sites responsible for survivin‑induced activation of NF‑κB p65. The present study demonstrated that the expression of survivin is positively correlated with IKKα and IKKβ in ESCC tissues. Survivin affected the mRNA and protein expression levels of IKKα, IKKβ, and NF‑κB p65 in Eca109 and KYSE150 cells. Furthermore, survivin increased the transcriptional activity of the IKKβ promoter and bound to the IKKβ promoter region in the Eca109 cells. Downregulation of survivin arrested the cell cycle at the G2/M phase and induced apoptosis. Results of the present study suggest that survivin activates NF‑κB p65 in Eca109 cells via binding to the IKKβ promoter region and upregulating IKKβ promoter transcriptional activity. Survivin overexpression activates NF‑κB p65, which is important in the acquisition and maintenance of the oncogenic characteristics of ESCC.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zeng W, Li H, Chen Y, Lv H, Liu L, Ran J, Sun X, Bieerkehazhi S, Liu Y, Li X, Li X, et al: Survivin activates NF‑κB p65 via the IKKβ promoter in esophageal squamous cell carcinoma. Mol Med Rep 13: 1869-1880, 2016
APA
Zeng, W., Li, H., Chen, Y., Lv, H., Liu, L., Ran, J. ... Li, H. (2016). Survivin activates NF‑κB p65 via the IKKβ promoter in esophageal squamous cell carcinoma. Molecular Medicine Reports, 13, 1869-1880. https://doi.org/10.3892/mmr.2015.4737
MLA
Zeng, W., Li, H., Chen, Y., Lv, H., Liu, L., Ran, J., Sun, X., Bieerkehazhi, S., Liu, Y., Li, X., Lai, W., Watibieke, J., Dawulietihan, M., Li, X., Li, H."Survivin activates NF‑κB p65 via the IKKβ promoter in esophageal squamous cell carcinoma". Molecular Medicine Reports 13.2 (2016): 1869-1880.
Chicago
Zeng, W., Li, H., Chen, Y., Lv, H., Liu, L., Ran, J., Sun, X., Bieerkehazhi, S., Liu, Y., Li, X., Lai, W., Watibieke, J., Dawulietihan, M., Li, X., Li, H."Survivin activates NF‑κB p65 via the IKKβ promoter in esophageal squamous cell carcinoma". Molecular Medicine Reports 13, no. 2 (2016): 1869-1880. https://doi.org/10.3892/mmr.2015.4737