Hypomethylation effects of curcumin, demethoxycurcumin and bisdemethoxycurcumin on WIF-1 promoter in non-small cell lung cancer cell lines

  • Authors:
    • Yu-Liang Liu
    • He-Ping Yang
    • Liang Gong
    • Chun-Lan Tang
    • Hai-Jing Wang
  • View Affiliations

  • Published online on: April 11, 2011     https://doi.org/10.3892/mmr.2011.473
  • Pages: 675-679
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Abstract

The tumor suppressor gene Wnt inhibitory factor-1 (WIF-1) has been found to be promoter hypermethylated and silenced in lung cancer cell lines and tissues. Curcuminoids are major active components of the spice turmeric, and have recently been reported to be potential hypomethylation agents. In the present study, the hypomethylation effects of three major curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin, were compared in vitro using ELISA, and their demethylation potential was confirmed by methylation-specific PCR. It was found that bisdemethoxycurcumin possesses the strongest demethylation function in vitro compared to the other two curcuminoids, exerting its effect at a minimal demethylation concentration of 0.5-1 µM. The WIF-1 promoter region was demethylated after treatment with 20 µM demethoxycurcumin and bisdemethoxycurcumin, but failed to respond to 20 µM curcumin. In the A549 cell line, RT-PCR and Western blotting were used to confirm that WIF-1 expression was restored after curcuminoid-induced promoter hypermethylation. Since the results regarding the demethylation potential of the three major curcuminoids to restore WIF-1 expression indicated that bisdemethoxycurcumin has the strongest hypomethylation effect, this curcuminoid may have therapeutic use in the restoration of WIF-1 expression in NSCLC.

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July-August 2011
Volume 4 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu Y, Yang H, Gong L, Tang C and Wang H: Hypomethylation effects of curcumin, demethoxycurcumin and bisdemethoxycurcumin on WIF-1 promoter in non-small cell lung cancer cell lines. Mol Med Rep 4: 675-679, 2011
APA
Liu, Y., Yang, H., Gong, L., Tang, C., & Wang, H. (2011). Hypomethylation effects of curcumin, demethoxycurcumin and bisdemethoxycurcumin on WIF-1 promoter in non-small cell lung cancer cell lines. Molecular Medicine Reports, 4, 675-679. https://doi.org/10.3892/mmr.2011.473
MLA
Liu, Y., Yang, H., Gong, L., Tang, C., Wang, H."Hypomethylation effects of curcumin, demethoxycurcumin and bisdemethoxycurcumin on WIF-1 promoter in non-small cell lung cancer cell lines". Molecular Medicine Reports 4.4 (2011): 675-679.
Chicago
Liu, Y., Yang, H., Gong, L., Tang, C., Wang, H."Hypomethylation effects of curcumin, demethoxycurcumin and bisdemethoxycurcumin on WIF-1 promoter in non-small cell lung cancer cell lines". Molecular Medicine Reports 4, no. 4 (2011): 675-679. https://doi.org/10.3892/mmr.2011.473