hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas

  • Authors:
    • Yi Wang
    • Hongmei Luo
    • Yangle Li
    • Tieding Chen
    • Shengpeng Wu
    • Luoyan Yang
  • View Affiliations

  • Published online on: October 11, 2011     https://doi.org/10.3892/mmr.2011.621
  • Pages: 260-265
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Abstract

Numerous microRNAs (miRNAs) play crucial roles in cancer development. In this study, we report that hsa-miR-96 is expressed at higher levels in human bladder urothelial carcinomas compared to normal tissues. We found that hsa-miR-96 increased invasion and differentiation of human bladder T24 cells and promoted their growth. Down‑regulation of hsa-miR-96 significantly affected the phenotype of bladder cancer T24 cells. The mRNA and protein levels of insulin receptor substrate 1 (IRS1) and MAP4K1 were significantly reduced in cells transfected with the hsa-miR-96 inhibitor when compared with levels in cells transfected with the empty plasmid vector or the negative control miRNA inhibitor. Altogether, these results suggest that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating IRS1 and MAP4K1 levels, functioning as a promising diagnostic marker in human bladder urothelial carcinomas.

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January 2012
Volume 5 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang Y, Luo H, Li Y, Chen T, Wu S and Yang L: hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas. Mol Med Rep 5: 260-265, 2012
APA
Wang, Y., Luo, H., Li, Y., Chen, T., Wu, S., & Yang, L. (2012). hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas. Molecular Medicine Reports, 5, 260-265. https://doi.org/10.3892/mmr.2011.621
MLA
Wang, Y., Luo, H., Li, Y., Chen, T., Wu, S., Yang, L."hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas". Molecular Medicine Reports 5.1 (2012): 260-265.
Chicago
Wang, Y., Luo, H., Li, Y., Chen, T., Wu, S., Yang, L."hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas". Molecular Medicine Reports 5, no. 1 (2012): 260-265. https://doi.org/10.3892/mmr.2011.621