Open Access

Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas

  • Authors:
    • Hiroko Kita
    • Keisei Okamoto
    • Ryoji Kushima
    • Akihiro Kawauchi
    • Tokuhiro Chano
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  • Published online on: June 2, 2015     https://doi.org/10.3892/ol.2015.3306
  • Pages: 661-666
  • Copyright: © Kita et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dimethyl sulfoxide (DMSO) is an amphipathic molecule that is used as a solvent in biological studies and as a vehicle for drug therapy. The present study was designed to evaluate the potential effects of DMSO as a solvent in the treatment of testicular embryonal carcinomas (ECs). DMSO was applied to two human EC cell lines (NEC8 and NEC14), with the treated cells evaluated in relation to cisplatin (CDDP) resistance, differentiation (using Vimentin, Fibronectin, TRA‑1‑60, and SSEA‑1 and ‑3 as markers) and stemness (denoted by expression of SOX2 and OCT3/4). Furthermore, DNA methyltransferase (DNMT‑1, ‑3A and ‑3L) expression and methylation status were analyzed. DMSO induced resistance to CDDP, aberrant differentiation and reduction of stemness‑related markers in each of the EC cell lines. The expression levels of DNMT‑3L and ‑3A were reduced in response to DMSO, while this treatment also affected DNA methylation. The data demonstrated that DMSO perturbed differentiation, reduced stemness and induced resistance to CDDP in human EC cells. Therefore, DMSO could reduce drug efficacy against EC cells and its use should be carefully managed in the clinical application of chemotherapy.
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August-2015
Volume 10 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kita H, Okamoto K, Kushima R, Kawauchi A and Chano T: Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncol Lett 10: 661-666, 2015
APA
Kita, H., Okamoto, K., Kushima, R., Kawauchi, A., & Chano, T. (2015). Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncology Letters, 10, 661-666. https://doi.org/10.3892/ol.2015.3306
MLA
Kita, H., Okamoto, K., Kushima, R., Kawauchi, A., Chano, T."Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas". Oncology Letters 10.2 (2015): 661-666.
Chicago
Kita, H., Okamoto, K., Kushima, R., Kawauchi, A., Chano, T."Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas". Oncology Letters 10, no. 2 (2015): 661-666. https://doi.org/10.3892/ol.2015.3306