Open Access

JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells

  • Authors:
    • Wen Li
    • Chuangyu Wen
    • Haiyan Bai
    • Xiaoyan Wang
    • Xiaoli Zhang
    • Lanlan Huang
    • Xiangling Yang
    • Aikichi Iwamoto
    • Huanliang Liu
  • View Affiliations

  • Published online on: June 11, 2015     https://doi.org/10.3892/ol.2015.3371
  • Pages: 709-715
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Piperlongumine (PPLGM), an alkaloid isolated from the long pepper (Piper longum L.), can selectively trigger cancer cell death in colorectal cancer cells. The present study investigated whether the c‑Jun NH2‑terminal kinase (JNK) signaling pathway is involved in PPLGM‑induced apoptosis in the human colorectal cancer HCT116 cell line. The results demonstrated that PPLGM reduced the cell viability and induced cell apoptosis in a time‑ and concentration‑dependent manner, without a significant effect on cell cycle distribution. Meanwhile, treatment with 10 µM PPLGM resulted in JNK activation within 1 h, and a marked and sustained increase in c‑Jun phosphorylation in the HCT116 cells. In addition, SP600125, a general inhibitor of JNK, inhibited PPLGM‑induced apoptosis in the HCT116 cells by inhibiting PPLGM‑induced c‑Jun phosphorylation. Altogether, it can be concluded that the JNK signaling pathway, at least in part, is involved in PPLGM‑mediated apoptosis in HCT116 cells.
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August-2015
Volume 10 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li W, Wen C, Bai H, Wang X, Zhang X, Huang L, Yang X, Iwamoto A and Liu H: JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells. Oncol Lett 10: 709-715, 2015
APA
Li, W., Wen, C., Bai, H., Wang, X., Zhang, X., Huang, L. ... Liu, H. (2015). JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells. Oncology Letters, 10, 709-715. https://doi.org/10.3892/ol.2015.3371
MLA
Li, W., Wen, C., Bai, H., Wang, X., Zhang, X., Huang, L., Yang, X., Iwamoto, A., Liu, H."JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells". Oncology Letters 10.2 (2015): 709-715.
Chicago
Li, W., Wen, C., Bai, H., Wang, X., Zhang, X., Huang, L., Yang, X., Iwamoto, A., Liu, H."JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells". Oncology Letters 10, no. 2 (2015): 709-715. https://doi.org/10.3892/ol.2015.3371