Open Access

Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells

  • Authors:
    • Ju‑Hee Lee
    • Chul Hong Park
    • Kwang‑Hoon Chun
    • Soon‑Sun Hong
  • View Affiliations

  • Published online on: June 10, 2015     https://doi.org/10.3892/ol.2015.3360
  • Pages: 730-736
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Adipose-derived stem cells (ASCs) are a population of cells derived from adipose tissue. ASCs exhibit multilineage development potential and are able to secrete various factors, which influence adjacent cells. Previous studies have reported the effectiveness of ASC‑conditioned medium (ASC‑CM) in wound healing, anti‑melanogenesis, wrinkle improvement and hair growth. In the present study, the anticancer function of ASC‑CM was investigated in vitro and in vivo. An MTT assay revealed that ASC‑CM significantly decreased the proliferation of B16 melanoma cells in a time‑ and dose‑dependent manner (P<0.01). Cell cycle analysis indicated that ASC‑CM significantly increased the number of cells in G1 phase while reducing the number of cells in the S and G2/M phases (P<0.01). Furthermore, a wound migration model demonstrated that ASC‑CM treatment significantly decreased the migration ability of B16 melanoma cells (P<0.01). In addition, C57BL/6 mice were administered with a single intratumoral injection of ASC‑CM, daily or every other day, and a significant reduction in the volume of the tumor mass was observed compared with that of the control group (P<0.01). Thus, the findings of the present study indicated that ASC‑CM has an anti‑tumorigenic effect on B16 melanoma cells in vitro and in vivo, and may potentially be used to support the treatment of melanoma in the future.
View Figures
View References

Related Articles

Journal Cover

August-2015
Volume 10 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee JH, Park CH, Chun KH and Hong SS: Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells. Oncol Lett 10: 730-736, 2015
APA
Lee, J., Park, C.H., Chun, K., & Hong, S. (2015). Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells. Oncology Letters, 10, 730-736. https://doi.org/10.3892/ol.2015.3360
MLA
Lee, J., Park, C. H., Chun, K., Hong, S."Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells". Oncology Letters 10.2 (2015): 730-736.
Chicago
Lee, J., Park, C. H., Chun, K., Hong, S."Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells". Oncology Letters 10, no. 2 (2015): 730-736. https://doi.org/10.3892/ol.2015.3360