Open Access

Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures

  • Authors:
    • Lorenz Kadletz
    • Gregor Heiduschka
    • Julian Domayer
    • Rainer Schmid
    • Elisabeth Enzenhofer
    • Dietmar Thurnher
  • View Affiliations

  • Published online on: July 14, 2015     https://doi.org/10.3892/ol.2015.3487
  • Pages: 1281-1286
  • Copyright: © Kadletz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Two-dimensional (2D) monolayer cell culture models are the most common method used to investigate tumor cells in vitro. In the few last decades, a multicellular spheroid model has gained attention due to its adjacency to tumors in vivo. The aim of the present study was to investigate immunohistochemical differences between these two cell culture systems. The FaDu, CAL27 and SCC25 head and neck squamous cell carcinoma (HNSCC) cell lines were seeded out in monolayer and multicellular spheroids. The FaDu and SCC25 cells were treated with increasing doses of cisplatin and irradiation. CAL27 cells were not used in theproliferation experiments, since the spheroids of CAL27 cells were not able to process the reagent in CCK-8 assays. Furthermore, they were stained to present alterations of the following antigens: Ki‑67, vascular endothelial growth factor receptor, epithelial growth factor and survivin. Differences in growth rates and expression patterns were detected in certain HNSCC cell lines. The proliferation rates showed a significant divergence of cells grown in the three‑dimensional model compared with cells grown in the 2D model. Overall, multicellular spheroids are a promising method to reproduce the immunohistochemical aspects and characteristics of tumor cells, and may show different response rates to therapeutic options.
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September-2015
Volume 10 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kadletz L, Heiduschka G, Domayer J, Schmid R, Enzenhofer E and Thurnher D: Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures. Oncol Lett 10: 1281-1286, 2015
APA
Kadletz, L., Heiduschka, G., Domayer, J., Schmid, R., Enzenhofer, E., & Thurnher, D. (2015). Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures. Oncology Letters, 10, 1281-1286. https://doi.org/10.3892/ol.2015.3487
MLA
Kadletz, L., Heiduschka, G., Domayer, J., Schmid, R., Enzenhofer, E., Thurnher, D."Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures". Oncology Letters 10.3 (2015): 1281-1286.
Chicago
Kadletz, L., Heiduschka, G., Domayer, J., Schmid, R., Enzenhofer, E., Thurnher, D."Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures". Oncology Letters 10, no. 3 (2015): 1281-1286. https://doi.org/10.3892/ol.2015.3487