Overexpression and lack of copy number variation in the BMI‑1 gene in human glioma

  • Authors:
    • Sibin Madathan Kandy
    • Dhananjaya Ishwara Bhat
    • Lavanya Choppavarapu
    • Arati Suvatha
    • Chetan Ghati Kasturirangan
  • View Affiliations

  • Published online on: September 8, 2015     https://doi.org/10.3892/ol.2015.3686
  • Pages: 3318-3322
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Abstract

Malignant gliomas are neoplasms of the brain that are associated with a poor prognosis. The B‑cell‑specific Moloney murine leukemia virus integration site 1 (BMI‑1) gene is one of the major cancer stem cell factors responsible for treatment failure in glioma. In the present study, the DNA‑RNA‑protein alterations in the BMI‑1 gene were assessed in 50 glioma samples. Copy number variations in the BMI‑1 gene were analyzed using SYBR® Green quantitative polymerase chain reaction. Gene expression analysis was performed using a Taqman assay and protein quantitation was performed using western blotting. A comparative Ct analysis showed the absence of copy number variations in all glioma samples. BMI‑1 mRNA expression was found to be overexpressed in 36 out of 50 samples (72.0%), and 37 out of 50 samples showed overexpression (74.0%) of BMI‑1 protein; this was statistically significant when compared with non‑glioma tissues. It was observed that the protein and RNA expression in glioma were concordant. In this study on the BMI‑1 gene, transcription and translation in glioma were observed and BMI‑1 overexpression was found to be a common phenomenon.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Madathan Kandy S, Ishwara Bhat D, Choppavarapu L, Suvatha A and Ghati Kasturirangan C: Overexpression and lack of copy number variation in the BMI‑1 gene in human glioma. Oncol Lett 10: 3318-3322, 2015
APA
Madathan Kandy, S., Ishwara Bhat, D., Choppavarapu, L., Suvatha, A., & Ghati Kasturirangan, C. (2015). Overexpression and lack of copy number variation in the BMI‑1 gene in human glioma. Oncology Letters, 10, 3318-3322. https://doi.org/10.3892/ol.2015.3686
MLA
Madathan Kandy, S., Ishwara Bhat, D., Choppavarapu, L., Suvatha, A., Ghati Kasturirangan, C."Overexpression and lack of copy number variation in the BMI‑1 gene in human glioma". Oncology Letters 10.5 (2015): 3318-3322.
Chicago
Madathan Kandy, S., Ishwara Bhat, D., Choppavarapu, L., Suvatha, A., Ghati Kasturirangan, C."Overexpression and lack of copy number variation in the BMI‑1 gene in human glioma". Oncology Letters 10, no. 5 (2015): 3318-3322. https://doi.org/10.3892/ol.2015.3686