Knockdown of protein phosphatase 5 inhibits ovarian cancer growth in vitro

  • Authors:
    • Xiaojiao Zheng
    • Lianxiao Zhang
    • Bohong Jin
    • Fubin Zhang
    • Duoyi Zhang
    • Lining Cui
  • View Affiliations

  • Published online on: October 26, 2015     https://doi.org/10.3892/ol.2015.3828
  • Pages: 168-172
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Ovarian cancer is the most common cause of gynecological cancer-related mortality. Serine/threonine protein phosphatase 5 (PP5, PPP5C) has been recognized to be involved in the regulation of multiple cellular signaling cascades that control diverse cellular processes, including cell growth, differentiation, proliferation, motility and apoptosis. In this study, to evaluate the functional role of PP5 in ovarian cancer cells, lentivirus‑mediated RNA interference (RNAi) was applied to silence PPP5C in the human ovarian cancer cell line CAOV‑3. Cell viability was measured by 3‑(4,5‑dimethyl­thiazol‑2‑yl)-2,5‑diphenyltetrazolium bromide assay. Cell colony forming ability was measured by colony formation. Cell cycle progression was determined by propidium iodide staining and flow cytometry. The results demonstrated that lentivirus-mediated RNAi specifically suppressed the expression of PPP5C at the mRNA and protein levels in CAOV‑3 cells. Further investigations revealed that PP5 knockdown significantly inhibited the proliferation and colony formation of CAOV‑3 cells. Moreover, the cell cycle of CAOV‑3 cells was arrested at the G0/G1 phase following PP5 knockdown. This study highlights the crucial role of PP5 in promoting ovarian cancer cell proliferation, and provides a foundation for further study into the clinical potential of lentiviral-mediated delivery of PP5 RNAi therapy for the treatment of ovarian cancer.
View Figures
View References

Related Articles

Journal Cover

January-2016
Volume 11 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zheng X, Zhang L, Jin B, Zhang F, Zhang D and Cui L: Knockdown of protein phosphatase 5 inhibits ovarian cancer growth in vitro. Oncol Lett 11: 168-172, 2016
APA
Zheng, X., Zhang, L., Jin, B., Zhang, F., Zhang, D., & Cui, L. (2016). Knockdown of protein phosphatase 5 inhibits ovarian cancer growth in vitro. Oncology Letters, 11, 168-172. https://doi.org/10.3892/ol.2015.3828
MLA
Zheng, X., Zhang, L., Jin, B., Zhang, F., Zhang, D., Cui, L."Knockdown of protein phosphatase 5 inhibits ovarian cancer growth in vitro". Oncology Letters 11.1 (2016): 168-172.
Chicago
Zheng, X., Zhang, L., Jin, B., Zhang, F., Zhang, D., Cui, L."Knockdown of protein phosphatase 5 inhibits ovarian cancer growth in vitro". Oncology Letters 11, no. 1 (2016): 168-172. https://doi.org/10.3892/ol.2015.3828