Disappearance of giant cells and presence of newly formed bone in the pulmonary metastasis of a sacral giant‑cell tumor following denosumab treatment: A case report

  • Authors:
    • Tetsuro Yamagishi
    • Hiroyuki Kawashima
    • Akira Ogose
    • Taro Sasaki
    • Tetsuo Hotta
    • Shoichi Inagawa
    • Hajime Umezu
    • Naoto Endo
  • View Affiliations

  • Published online on: November 4, 2015     https://doi.org/10.3892/ol.2015.3858
  • Pages: 243-246
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Abstract

A giant‑cell tumor of the bone (GCTB) is a benign but locally aggressive bone tumor. Recently, the receptor activator of nuclear factor κB (RANK) ligand inhibitor, denosumab, has demonstrated activity against giant‑cell tumors. The current study reports a case of a sacral GCTB with lung metastasis. A 19‑year‑old male patient presented with right buttock pain and right lower leg pain, and a sacral GCTB was diagnosed based on the histological analysis of a biopsy specimen. The patient was successfully treated with neoadjuvant denosumab therapy, which allowed curettage. In addition, the pulmonary nodule reduced in size following denosumab administration, and surgical resection was performed. Since the operation, the patient has been managed with the continued use of denosumab with no sign of recurrence. Microscopic findings from the surgical specimen following denosumab treatment revealed that the giant cells had disappeared and woven bone had formed. The specimen from the pulmonary nodule exhibited similar findings to the surgical specimen. It was reported that denosumab treatment was able to reduce the number of giant cells and RANK‑positive stromal cells, and cause the formation of new bone in the primary lesion. The present study reports the first case to demonstrate the efficiency of denosumab in treating pulmonary metastasis of GCTB.

Introduction

A giant-cell tumor of the bone (GCTB) is a benign but aggressive skeletal neoplasm, with a high recurrence rate (1,2) of between 33 and 50% (3,4). Therefore, it is desirable to perform en bloc resection as a radical cure. However, surgical interventions, including curettage and resection, are associated with significant complications and recurrence rates. The treatment of GCTB is controversial (3,5). Partial and/or total sacrectomy is the ideal curative treatment. However, GCTB in the spine, pelvis and sacrum present as massive tumors and the size and location make surgical treatment difficult and dangerous (5). It can result in significant morbidity, including neurological complication and infection, and mortality (6). In addition, GCTBs have abundant vessels that often cause massive bleeding during surgery (3). Therefore, it is difficult to treat GCTBs of the sacrum. The survival rate to recurrence is 90% at 60 and 120 months (7). Survival to local recurrence with and without radiation was 91% and 86%, with and without embolization was 91% and 86%, and with and without local adjuvants 88% and 92%, respectively (7). However, a number of treatment modalities have been reported. The standard treatment is aggressive curettage or wide resection. If complete resection cannot be easily achieved, preoperative arterial embolization, intralesional curettage, or radiation therapy are alternatives (7). However, the best treatment for GCTB of the sacrum remains controversial and unclear. Lung metastasis from a GCTB is rare; it is reportedly observed in 2–10% of patients with GCTB (8,9).

Denosumab is a fully human monoclonal antibody against the receptor activator of nuclear factor κB (RANK) ligand (RANKL). RANKL facilitates the differentiation of osteoclast precursors into osteoclasts, resulting in bone resorption (10). This is important in the regulation of bone growth and turnover. In a phase II study of adults with giant-cell tumors, significant clinical response to denosumab was observed (11): The clinical response refers to the elimination of giant cells histologically in 20/35 patients who were administrated denosumab. Denosumab was approved for the treatment of GCTBs in June 2014 in Japan. The effect of denosumab treatment on patients with GCTBs has previously been reported; 90% of GCTB patients derived clinical benefit from denosumab, such as functional status or pain (12).

The current study presents the case of a sacral GCTB with pulmonary metastasis, which was treated with curettage and denosumab. This case demonstrates the effect of denosumab on pulmonary metastasis from GCTB.

Case report

A 19-year-old male patient presented to Niigata University Medical and Dental Hospital (Niigata, Japan) in January 2013 complaining of right buttock pain and right lower leg pain. Initial magnetic resonance imaging revealed a bone tumor in the sacrum (Fig. 1). Computed tomography (CT) scans of the sacrum (Fig. 2) and lungs (Fig. 3) were performed. This revealed an osteolytic lesion in the sacrum (Fig. 2A). In addition, enhanced chest CT revealed a right pulmonary nodule, measuring 6 mm in diameter, which was suspected to be metastasis from GCTB (Fig. 3A). An open biopsy of the sacrum was performed, and analysis of the specimen revealed that it consisted of stromal cells and multinucleated osteoclast giant cells, leading to a diagnosis of GCTB (Fig. 4). Initially, the treatment plan included preoperative embolization and surgical treatment. Embolization with polyvinyl alcohol was performed twice. Following embolization, the buttock pain improved.

The patient subsequently received 120 mg of denosumab once per month, commencing in January 2013, as neoadjuvant therapy for the sacral tumor and the pulmonary nodule. Follow-up CT after 8 months of treatment, revealed gradual osteogenesis in the sacral tumor over time (Fig. 2B). In September 2013, follow-up CT revealed marked osteogenesis, and the tumor was judged to be stable; surgery was subsequently planned (Fig. 2C). Following preoperative embolization with micro-coils (Tornado®; Kook Japan, Tokyo, Japan), curettage of the tumor was undertaken with laminectomy at the L5-S1 spinal segments. Histopathological examination revealed an immature trabecular bone, and osteoclast-type giant cells were not observed in the sacral specimen (Fig. 5).

Following surgery, the patient has continued to receive 120 mg denosumab once per month. After 10 months of denosumab treatment, a chest CT scan (performed in December 2013) indicated that the pulmonary nodule had reduced in size sufficiently to allow curative surgery, which was subsequently performed in January 2014 (Fig. 3B). The nodule was resected via video-assisted thoracoscopic surgery. Histopathologically, osteoclast-type giant cells or spindle cells were not observed in the pulmonary nodule, while osteoids were observed (Fig. 6). At the follow-up examination 6 months later, CT imaging did not show any tumor progression; however, ossification was observed in the sacrum. This was considered to be an effect of the denosumab treatment. The patient is currently 20 months into the treatment, and has no buttock pain. The patient has received 120 mg/month denosumab after surgical treatment. Present CT scan revealed no evidence of recurrence. Further treatment is not planned at present, however the interval of denosumab administration may be extended according to symptoms or follow up CT scans in the future.

Discussion

Systemic treatment with denosumab as neoadjuvant therapy has been demonstrated to successfully reduce the surgical morbidity of tumor resection, and leads to osteoclast absence and reactive bone formation (13). In the present study, curettage of the GCTB was conducted following denosumab neoadjuvant therapy, with a small amount of bleeding. Denosumab exerts its effects by binding to RANKL, thus blocking its binding to RANK on osteoclasts and osteoclast precursors. This inhibits the differentiation of osteoclasts and osteoclast-mediated bone resorption. As the giant cells in GCTB express RANK, denosumab is a promising target-specific therapy for this tumor (14,15). Thomas et al (11) reported the result of an open-label, phase II study with single-agent denosumab in patients who were aged >18 years and had recurrent giant-cell tumors or unresectable tumors. Following 25 weeks of treatment, 86% of patients met tumor response criteria (11).

Branstetter et al (12) reported that denosumab treatment for patients with GCTBs significantly reduced RANK-positive giant cells and stromal cells, and promoted bone formation at the primary lesion. In the present study, compared with the open biopsy and resected sacral specimens, giant cells disappeared following denosumab treatment, and bone formation was observed. Furthermore, the chest CT scan revealed that the pulmonary nodule reduced in size following denosumab treatment; the nodule from which the pathological examination specimen was taken demonstrated bone formation, while giant cells were not observed in the pulmonary specimen. Akaike et al (16) reported local recurrence of a GCTB following treatment with denosumab, in which bone formation was observed histologically and multinucleated giant cells had disappeared. Histological changes following denosumab treatment were reported in the soft tissue of the GCTB, but not in the pulmonary metastasis from GCTB. The case descibed by Akaike et al (16) was the first case to demonstrate the efficiency of denosumab in treating a pulmonary metastasis from GCTB.

In the present study, diagnosis of the pulmonary lesion as metastasis from GCTB was difficult; the differential diagnoses of this nodule included metastasis from GCTB, osteosarcoma and undifferentiated pleomorphic sarcoma. As giant cells were not observed and stromal cells exhibited little cytological atypia in the pulmonary specimen, the bone formation in the pulmonary nodule was thought to be non-neoplastic but rather reactive bone formation and, therefore, the pulmonary nodule was diagnosed as a metastasis of the GCTB.

Certain patients may require long-term denosumab treatment; however its toxicity profile raises concerns regarding long-term use (13). The optimal duration of denosumab treatment is unknown. In addition, Skubitz et al (17) reported that recurrences following apparent complete responses have been observed after the discontinuation of denosumab treatment. Another problem is monitoring the patient for complications and recurrence after discontinuing the administration of denosumab (14,18). Long-term follow-up is necessary as long-term treatment has not been attempted previously. Denosumab may be an effective option in cases in which surgical treatment is associated with high morbidity.

In conclusion, denosumab suppresses osteoclast differentiation and reduces bone resorption. In the present study, denosumab demonstrated efficacy against pulmonary metastasis of GCTB. Microscopic findings from pulmonary specimens following denosumab treatment revealed the disappearance of giant cells and the presence of newly formed bone.

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Spandidos Publications style
Yamagishi T, Kawashima H, Ogose A, Sasaki T, Hotta T, Inagawa S, Umezu H and Endo N: Disappearance of giant cells and presence of newly formed bone in the pulmonary metastasis of a sacral giant‑cell tumor following denosumab treatment: A case report. Oncol Lett 11: 243-246, 2016
APA
Yamagishi, T., Kawashima, H., Ogose, A., Sasaki, T., Hotta, T., Inagawa, S. ... Endo, N. (2016). Disappearance of giant cells and presence of newly formed bone in the pulmonary metastasis of a sacral giant‑cell tumor following denosumab treatment: A case report. Oncology Letters, 11, 243-246. https://doi.org/10.3892/ol.2015.3858
MLA
Yamagishi, T., Kawashima, H., Ogose, A., Sasaki, T., Hotta, T., Inagawa, S., Umezu, H., Endo, N."Disappearance of giant cells and presence of newly formed bone in the pulmonary metastasis of a sacral giant‑cell tumor following denosumab treatment: A case report". Oncology Letters 11.1 (2016): 243-246.
Chicago
Yamagishi, T., Kawashima, H., Ogose, A., Sasaki, T., Hotta, T., Inagawa, S., Umezu, H., Endo, N."Disappearance of giant cells and presence of newly formed bone in the pulmonary metastasis of a sacral giant‑cell tumor following denosumab treatment: A case report". Oncology Letters 11, no. 1 (2016): 243-246. https://doi.org/10.3892/ol.2015.3858