Costimulation with anti-cluster of differentiation 3 and anti-cluster of differentiation 28 reduces the activity of mucin 1-stimulated human mononuclear cells

  • Authors:
    • Stephen E. Wright
    • Kathleen A. Rewers‑Felkins
    • Imelda Quinlin
    • Fatema Zohra
    • Jewel Ahmed
  • View Affiliations

  • Published online on: October 29, 2015     https://doi.org/10.3892/ol.2015.3840
  • Pages: 461-465
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Abstract

Cytotoxic T-lymphocyte activation and extension of the cell life span is necessary in order to enable immunotherapy to perform effectively against cancer. In the present study, mucin 1 (MUC1)-stimulated human mononuclear cells (M1SHMCs) were costimulated with bead-attached monoclonal antibodies specific for cluster of differentiation (CD)3 and CD28 receptors. The study was undertaken to determine whether costimulation was capable of enhancing the killing of cancer cells in vitro and of protecting non‑obese diabetic severe combined immunodeficient mice from tumor development. Lysis of MCF‑7 tumor cells by M1SHMCs was reduced following costimulation with anti‑CD3 and anti‑CD28. Furthermore, costimulation with anti‑CD3 and anti‑CD28 eliminated the protective effects of M1SHMCs on MCF‑7 breast cancer cell growth in the non‑obese diabetic severe combined immunodeficient mice. The present study suggested that costimulation with anti‑CD3 and anti‑CD28 is not advisable following antigen activation of lymphocytes under the conditions used here. Using a lower anti‑CD3/CD28 bead to T‑cell ratio may prevent immune suppression, however, further studies are required to support this hypothesis.
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January-2016
Volume 11 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wright SE, Rewers‑Felkins KA, Quinlin I, Zohra F and Ahmed J: Costimulation with anti-cluster of differentiation 3 and anti-cluster of differentiation 28 reduces the activity of mucin 1-stimulated human mononuclear cells. Oncol Lett 11: 461-465, 2016
APA
Wright, S.E., Rewers‑Felkins, K.A., Quinlin, I., Zohra, F., & Ahmed, J. (2016). Costimulation with anti-cluster of differentiation 3 and anti-cluster of differentiation 28 reduces the activity of mucin 1-stimulated human mononuclear cells. Oncology Letters, 11, 461-465. https://doi.org/10.3892/ol.2015.3840
MLA
Wright, S. E., Rewers‑Felkins, K. A., Quinlin, I., Zohra, F., Ahmed, J."Costimulation with anti-cluster of differentiation 3 and anti-cluster of differentiation 28 reduces the activity of mucin 1-stimulated human mononuclear cells". Oncology Letters 11.1 (2016): 461-465.
Chicago
Wright, S. E., Rewers‑Felkins, K. A., Quinlin, I., Zohra, F., Ahmed, J."Costimulation with anti-cluster of differentiation 3 and anti-cluster of differentiation 28 reduces the activity of mucin 1-stimulated human mononuclear cells". Oncology Letters 11, no. 1 (2016): 461-465. https://doi.org/10.3892/ol.2015.3840