Upregulation of ULK1 expression in PC‑3 cells following tumor protein P53 transfection by sonoporation

  • Authors:
    • Yu Wang
    • Yi‑Ni Chen
    • Wei Zhang
    • Yu Yang
    • Wen‑Kun Bai
    • E Shen
    • Bing Hu
  • View Affiliations

  • Published online on: November 18, 2015     https://doi.org/10.3892/ol.2015.3946
  • Pages: 699-704
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Abstract

The aim of the present study was to investigate whether ultrasound combined with microbubbles was able to enhance liposome‑mediated transfection of genes into human prostate cancer cells, and to examine the association between autophagy and tumor protein P53 (P53). An MTT assay was used to evaluate cell viability, while flow cytometry and fluorescence microscopy were used to measure gene transfection efficiency. Autophagy was observed using transmission electron microscopy. Reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis were used to assess the expression of autophagy‑associated genes. The results of the present study revealed that cell viability was significantly reduced following successfully enhanced transfection of P53 by ultrasound combined with microbubbles. In addition, serine/threonine‑protein kinase ULK1 levels were simultaneously upregulated. Castration‑resistant prostate cancer is difficult to treat and is investigated in the present study. P53 has a significant role in a number of key biological functions, including DNA repair, apoptosis, cell cycle, autophagy, senescence and angiogenesis. Prior to the present study, to the best of our knowledge, increased transfection efficiency and reduced side effects have been difficult to achieve. Ultrasound is considered to be a ‘gentle’ technique that may be able to achieve increased transfection efficiency and reduced side effects. The results of the present study highlight a potential novel therapeutic strategy for the treatment of prostate cancer.
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January-2016
Volume 11 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang Y, Chen YN, Zhang W, Yang Y, Bai WK, Shen E and Hu B: Upregulation of ULK1 expression in PC‑3 cells following tumor protein P53 transfection by sonoporation. Oncol Lett 11: 699-704, 2016
APA
Wang, Y., Chen, Y., Zhang, W., Yang, Y., Bai, W., Shen, E., & Hu, B. (2016). Upregulation of ULK1 expression in PC‑3 cells following tumor protein P53 transfection by sonoporation. Oncology Letters, 11, 699-704. https://doi.org/10.3892/ol.2015.3946
MLA
Wang, Y., Chen, Y., Zhang, W., Yang, Y., Bai, W., Shen, E., Hu, B."Upregulation of ULK1 expression in PC‑3 cells following tumor protein P53 transfection by sonoporation". Oncology Letters 11.1 (2016): 699-704.
Chicago
Wang, Y., Chen, Y., Zhang, W., Yang, Y., Bai, W., Shen, E., Hu, B."Upregulation of ULK1 expression in PC‑3 cells following tumor protein P53 transfection by sonoporation". Oncology Letters 11, no. 1 (2016): 699-704. https://doi.org/10.3892/ol.2015.3946