Hepatocyte growth factor increases the invasive potential of PC‑3 human prostate cancer cells via an ERK/MAPK and Zeb‑1 signaling pathway

  • Authors:
    • Yili Han
    • Yong Luo
    • Yongxing Wang
    • Yatong Chen
    • Mingchuan Li
    • Yongguang Jiang
  • View Affiliations

  • Published online on: November 18, 2015     https://doi.org/10.3892/ol.2015.3943
  • Pages: 753-759
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Abstract

Hepatocyte growth factor (HGF) has been implicated in epithelial‑mesenchymal transition (EMT) in numerous types of cancer. However, to the best of our knowledge, there has been no previous evidence that HGF has a role in prostate cancer. The present study aimed to investigate the effect of HGF on EMT and invasive potential, as well as the underlying molecular mechanisms, in a human prostate cancer cell line. Therefore, PC‑3 cells were treated with various concentrations of HGF for varying durations. EMT‑associated proteins, including E‑cadherin and vimentin, were examined by western blot analysis. The effects of HGF on cell proliferation, migration, invasion and tumorigenicity were assessed using MTT, wound‑healing, Transwell and soft‑agar assays. Subsequently, the role of c‑Met in the mediation of EMT‑like changes was investigated using reverse transcription‑polymerase chain reaction, western blot analysis and gene knockdown by small interfering RNA. Finally, western blot analysis was used to quantify the expression of a downstream transcription factor and extracellular signal‑related kinase/mitogen activated protein kinase (ERK/MAPK) signaling pathway proteins. The results indicated that treatment with HGF induced EMT‑like changes and enhanced the invasive potential of PC‑3 cells. There was an increase in the expression of ERK, phosphorylated‑ERK and zinc finger E‑box binding homeobox‑1 (Zeb‑1), suggesting that EMT‑like changes may be mediated through the ERK/MAPK and Zeb‑1 signaling pathway. Furthermore, HGF‑mediated EMT‑like changes were associated with c‑Met activation, and these changes were able to be blocked by c‑Met knockdown. The present study demonstrated that HGF‑induced EMT increased the invasive potential of PC‑3 human prostate cancer cells through activating the ERK/MAPK and Zeb‑1 signaling pathway.
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January-2016
Volume 11 Issue 1

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Spandidos Publications style
Han Y, Luo Y, Wang Y, Chen Y, Li M and Jiang Y: Hepatocyte growth factor increases the invasive potential of PC‑3 human prostate cancer cells via an ERK/MAPK and Zeb‑1 signaling pathway. Oncol Lett 11: 753-759, 2016
APA
Han, Y., Luo, Y., Wang, Y., Chen, Y., Li, M., & Jiang, Y. (2016). Hepatocyte growth factor increases the invasive potential of PC‑3 human prostate cancer cells via an ERK/MAPK and Zeb‑1 signaling pathway. Oncology Letters, 11, 753-759. https://doi.org/10.3892/ol.2015.3943
MLA
Han, Y., Luo, Y., Wang, Y., Chen, Y., Li, M., Jiang, Y."Hepatocyte growth factor increases the invasive potential of PC‑3 human prostate cancer cells via an ERK/MAPK and Zeb‑1 signaling pathway". Oncology Letters 11.1 (2016): 753-759.
Chicago
Han, Y., Luo, Y., Wang, Y., Chen, Y., Li, M., Jiang, Y."Hepatocyte growth factor increases the invasive potential of PC‑3 human prostate cancer cells via an ERK/MAPK and Zeb‑1 signaling pathway". Oncology Letters 11, no. 1 (2016): 753-759. https://doi.org/10.3892/ol.2015.3943