MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma

  • Authors:
    • Wen‑Zhou Ding
    • Qing‑Feng Ni
    • Ye‑Ting Lu
    • Liang‑Liang Kong
    • Jian‑Jun Yu
    • Long‑Wei Tan
    • Lian‑Bao Kong
  • View Affiliations

  • Published online on: December 1, 2015     https://doi.org/10.3892/ol.2015.3981
  • Pages: 1081-1088
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Abstract

Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver. MicroRNA-497 (miR-497) is known to be downregulated in several types of human cancer; however, the expression, function and underlying mechanisms of miR‑497 in HCC remain unclear. Therefore, the present study investigated miR‑497 expression in HCC samples and HCC‑derived cell lines using reverse transcription‑quantitative polymerase chain reaction. The protein expression of one of the predicted common targets of miR‑497, insulin‑like growth factor‑1 receptor (IGF‑1R), was assessed using western blot analyses and immunohistochemistry. The role of miR‑497 in regulating the proliferation of HCC‑derived cells was also investigated in vitro and in vivo. Of 60 paired specimens from HCC patients, miR‑497 was downregulated in 42 cancer specimens compared with adjacent non‑cancer tissues. Western blotting and immunohistochemical analyses revealed that IGF‑1R expression was significantly increased in HCC compared to control tissues. In addition, overexpression of miR‑497 was observed to inhibit colony formation and tumor growth in MHCC‑97H human HCC cells. Conversely, SMMC‑7721 human HCC cells transfected with a miR‑497 inhibitor exhibited enhanced colony formation and tumor growth. Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. These results indicate that miR-497 may be a potentially effective gene therapy target.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ding WZ, Ni QF, Lu YT, Kong LL, Yu JJ, Tan LW and Kong LB: MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma. Oncol Lett 11: 1081-1088, 2016
APA
Ding, W., Ni, Q., Lu, Y., Kong, L., Yu, J., Tan, L., & Kong, L. (2016). MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma. Oncology Letters, 11, 1081-1088. https://doi.org/10.3892/ol.2015.3981
MLA
Ding, W., Ni, Q., Lu, Y., Kong, L., Yu, J., Tan, L., Kong, L."MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma". Oncology Letters 11.2 (2016): 1081-1088.
Chicago
Ding, W., Ni, Q., Lu, Y., Kong, L., Yu, J., Tan, L., Kong, L."MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma". Oncology Letters 11, no. 2 (2016): 1081-1088. https://doi.org/10.3892/ol.2015.3981