Open Access

Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR

  • Authors:
    • Yazeed A. Al‑Sheikh
    • Hazem K. Ghneim
    • Khalil I. Softa
    • Abdulrahman A. Al‑Jobran
    • Omar Al‑Obeed
    • Mansoor A.V. Mohamed
    • Maha Abdulla
    • Mourad A.M. Aboul‑Soud
  • View Affiliations

  • Published online on: December 31, 2015     https://doi.org/10.3892/ol.2015.4068
  • Pages: 1406-1412
  • Copyright: © Al‑Sheikh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs or miRs) have been advocated as potentially robust and highly stable biomarkers of diverse disease conditions including cancer. The primary aim of this study was two‑fold: i) to profile the expression levels of selected mature miRNA signature genes, such as miR‑145, miR‑195, miR‑29 and miR‑92, in a paired‑study design of 20 colorectal cancer (CRC) tissues from patients versus adjacent neoplasm‑free mucosal tissues employing reverse transcription‑quantitative polymerase chain reaction; and ii) to examine their expression level in the plasma of the same CRC patients in relation to the age‑matched plasma of healthy controls. Statistically significant (P<0.01) increases in miR‑29 (2.5) and miR‑92 (2.6) were observed in CRC tissues compared with adjacent neoplasm‑free mucosal tissues. Profiling of CRC plasma samples showed that the expression levels of circulating miR‑29 and miR‑92 were significantly higher (P<0.01) than in the age‑matched normal plasma. By contrast, miR‑145 and miR‑195 exhibited significant (P<0.05) decreases in their mean expression levels in CRC tissue samples in relation to the normal tissues. The mean expression levels of miR‑145 and miR‑195 were significantly lower (P<0.05) in CRC plasma than the healthy controls. Distinct stage‑dependent changes in the expression level of the four miRNA gene profiles were observed between stages II and IV plasma of CRC patients relative to the control plasma. Taken together, the results clearly reflect a similar trend for the four miRNA expression levels in tissue and plasma as well as the positive correlation in the levels of miRNAs in tissues and plasma. These findings may be useful to clarify the molecular mechanisms underlying colorectal carcinogenesis and to underscore the potential of the investigated miRNAs as novel early diagnostic biomarkers of CRC.
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February-2016
Volume 11 Issue 2

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Spandidos Publications style
Al‑Sheikh YA, Ghneim HK, Softa KI, Al‑Jobran AA, Al‑Obeed O, Mohamed MA, Abdulla M and Aboul‑Soud MA: Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR. Oncol Lett 11: 1406-1412, 2016
APA
Al‑Sheikh, Y.A., Ghneim, H.K., Softa, K.I., Al‑Jobran, A.A., Al‑Obeed, O., Mohamed, M.A. ... Aboul‑Soud, M.A. (2016). Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR. Oncology Letters, 11, 1406-1412. https://doi.org/10.3892/ol.2015.4068
MLA
Al‑Sheikh, Y. A., Ghneim, H. K., Softa, K. I., Al‑Jobran, A. A., Al‑Obeed, O., Mohamed, M. A., Abdulla, M., Aboul‑Soud, M. A."Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR". Oncology Letters 11.2 (2016): 1406-1412.
Chicago
Al‑Sheikh, Y. A., Ghneim, H. K., Softa, K. I., Al‑Jobran, A. A., Al‑Obeed, O., Mohamed, M. A., Abdulla, M., Aboul‑Soud, M. A."Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR". Oncology Letters 11, no. 2 (2016): 1406-1412. https://doi.org/10.3892/ol.2015.4068