JF-305, a pancreatic cancer cell line is highly sensitive to the PARP inhibitor olaparib

  • Authors:
    • Xueli Yang
    • Charles Ndawula
    • Haiyan Zhou
    • Xiaohai Gong
    • Jian Jin
  • View Affiliations

  • Published online on: December 3, 2014     https://doi.org/10.3892/ol.2014.2762
  • Pages: 757-761
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Abstract

Poly(ADP‑ribose) polymerase‑1 (PARP‑1) is a DNA nick sensor involved in the base excision repair (BER) pathway. Olaparib, a PARP inhibitor, has demonstrated antitumor activity in homologous recombination (HR)‑deficient cancers. To extend this specific therapy to other types of carcinomas, a panel of 11 different cancer cells were screened in the present study. JF‑305, a pancreatic cancer cell line of Chinese origin, demonstrated sensitivity to the PARP inhibitor 6(5H)‑phenanthridinone. In the present study, 3 µM olaparib conferred a cell survival rate of 25% following four days of treatment. The colony formation efficiency was 83% at 10 nM, and dropped to 12% at 1 µM following seven days of treatment. Furthermore, olaparib induced cell cycle arrest in the S and G2/M phases prior to the initiation of apoptosis. Although the incidence of double‑strand breaks (DSBs) was increased in the olaparib‑treated JF-305 cells, the RAD51 foci were well formed at the sites of γ‑H2AX recruitment, indicating an activated HR mechanism. Furthermore, tumor growth was reduced by 49.8% following 22 days of consecutive administration of 10 mg/kg olaparib in the JF‑305 xenograft mouse model. In summary, the JF‑305 cell line was sensitive to olaparib and provided a prospective model for the preclinical assessment of PARP inhibitors in the therapy of pancreatic cancer.
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February-2015
Volume 9 Issue 2

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Copy and paste a formatted citation
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Spandidos Publications style
Yang X, Ndawula C, Zhou H, Gong X and Jin J: JF-305, a pancreatic cancer cell line is highly sensitive to the PARP inhibitor olaparib. Oncol Lett 9: 757-761, 2015
APA
Yang, X., Ndawula , C., Zhou, H., Gong, X., & Jin, J. (2015). JF-305, a pancreatic cancer cell line is highly sensitive to the PARP inhibitor olaparib. Oncology Letters, 9, 757-761. https://doi.org/10.3892/ol.2014.2762
MLA
Yang, X., Ndawula , C., Zhou, H., Gong, X., Jin, J."JF-305, a pancreatic cancer cell line is highly sensitive to the PARP inhibitor olaparib". Oncology Letters 9.2 (2015): 757-761.
Chicago
Yang, X., Ndawula , C., Zhou, H., Gong, X., Jin, J."JF-305, a pancreatic cancer cell line is highly sensitive to the PARP inhibitor olaparib". Oncology Letters 9, no. 2 (2015): 757-761. https://doi.org/10.3892/ol.2014.2762