MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells

  • Authors:
    • Chenghui Liu
    • Shixing Liang
    • Shenghui Xiao
    • Qiming Lin
    • Xu Chen
    • Yi Wu
    • Jian Fu
  • View Affiliations

  • Published online on: January 12, 2015     https://doi.org/10.3892/ol.2015.2865
  • Pages: 1393-1397
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Abstract

MicroRNA (miR)‑27b has been reported to participate in glioma. However, a detailed role of miR‑27b and the underlying mechanism remain largely unknown. The present study found that the expression of miR‑27b was significantly increased in glioma tissues compared with normal adjacent tissues. In addition, miR‑27b was also upregulated in the U87, U251 and SHG44 glioma cell lines compared with normal human astrocytes. Sprouty homolog 2 (Spry2), which has been reported to be associated with invasive glioma, was identified as a novel target of miR‑27b in U251 glioma cells, and the protein expression of Spry2 was negatively regulated by miR‑27b in U251 cells. Additionally, inhibition of miR‑27b and upregulation of Spry2 suppressed glioma cell invasion, while downregulation of Spry2 reversed the suppressive effect of miR‑27b inhibition on glioma cell invasion. These data suggest that miR‑27b may promote glioma cell invasion through direct inhibition of Spry2 expression. The data also suggest that miR‑27b may become a promising molecular target for inhibiting the invasion and metastasis of glioma.
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March-2015
Volume 9 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu C, Liang S, Xiao S, Lin Q, Chen X, Wu Y and Fu J: MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells. Oncol Lett 9: 1393-1397, 2015
APA
Liu, C., Liang, S., Xiao, S., Lin, Q., Chen, X., Wu, Y., & Fu, J. (2015). MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells. Oncology Letters, 9, 1393-1397. https://doi.org/10.3892/ol.2015.2865
MLA
Liu, C., Liang, S., Xiao, S., Lin, Q., Chen, X., Wu, Y., Fu, J."MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells". Oncology Letters 9.3 (2015): 1393-1397.
Chicago
Liu, C., Liang, S., Xiao, S., Lin, Q., Chen, X., Wu, Y., Fu, J."MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells". Oncology Letters 9, no. 3 (2015): 1393-1397. https://doi.org/10.3892/ol.2015.2865