LGR5 promotes the proliferation of colorectal cancer cells via the Wnt/β-catenin signaling pathway

  • Authors:
    • Yu Lin
    • Tingyu Wu
    • Qianqian Yao
    • Shuming Zi
    • Long Cui
    • Ming Yang
    • Jinming Li
  • View Affiliations

  • Published online on: April 23, 2015     https://doi.org/10.3892/ol.2015.3144
  • Pages: 2859-2863
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Abstract

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) is an established cancer stem cell marker and is a target gene of the Wnt/β‑catenin signaling pathway, a critical pathway in the process of tumor initiation and growth. In the present study, the mRNA expression levels of LGR5, adenomatous polyposis coli (APC) and β‑catenin were detected in 20 colorectal cancer (CRC) tissues and matched healthy mucosa samples using reverse transcription‑quantitative polymerase chain reaction. HT-29 CRC cell line was treated with siRNA-Lgr5; the APC, β-catenin and LGR5 RNA expressions were detected and cell viability was measured using a CCK8 assay. The results revealed that LGR5 was significantly overexpressed in CRC tissue compared with healthy mucosa (P<0.05). Furthermore, knockdown of LGR5 by small interfering RNA decreased the expression of APC and β‑catenin in HT29 colon cancer cells as well as inhibited the proliferation of HT29 cells. These findings demonstrated that LGR5 expression is critical for the promotion of neoplastic CRC cell proliferation, indicating that LGR5 may be a novel therapeutic target for CRC.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lin Y, Wu T, Yao Q, Zi S, Cui L, Yang M and Li J: LGR5 promotes the proliferation of colorectal cancer cells via the Wnt/β-catenin signaling pathway. Oncol Lett 9: 2859-2863, 2015
APA
Lin, Y., Wu, T., Yao, Q., Zi, S., Cui, L., Yang, M., & Li, J. (2015). LGR5 promotes the proliferation of colorectal cancer cells via the Wnt/β-catenin signaling pathway. Oncology Letters, 9, 2859-2863. https://doi.org/10.3892/ol.2015.3144
MLA
Lin, Y., Wu, T., Yao, Q., Zi, S., Cui, L., Yang, M., Li, J."LGR5 promotes the proliferation of colorectal cancer cells via the Wnt/β-catenin signaling pathway". Oncology Letters 9.6 (2015): 2859-2863.
Chicago
Lin, Y., Wu, T., Yao, Q., Zi, S., Cui, L., Yang, M., Li, J."LGR5 promotes the proliferation of colorectal cancer cells via the Wnt/β-catenin signaling pathway". Oncology Letters 9, no. 6 (2015): 2859-2863. https://doi.org/10.3892/ol.2015.3144