TOPOLOGICAL AND QUANTITATIVE-ANALYSES OF EARLY EVENTS IN TUMOR-FORMATION USING HISTOCHEMICALLY-TAGGED TRANSFORMED 3T3 CELLS

  • Authors:
    • KL OCONNOR
    • LA CULP
  • View Affiliations

  • Published online on: Thursday, September 1, 1994
  • Pages: 869-876
  • DOI: 10.3892/or.1.5.869

Abstract

Human EJ H-ras or c-sis oncogene-transformed Balb/c 3T3 cells were investigated to discern the oncogene dependence of earliest events of primary tumor development by utilizing bacterial lacZ or human alkaline phosphatase histochemical marker genes. The locations and morphologies of transformed or untransformed Balb/c 3T3 cells, also transfected with either marker gene, were monitored in situ by visual, histochemical staining of skin after subcutaneous injection. Cell numbers remaining in the subcutaneous space were quantitated through newly-developed assays maximizing enzymatic detection of the transfected marker gene-encoded enzymes using luminescent substrates. Either oncogene was effective at facilitating cell establishment within the subcutaneous space while parental 3T3 cells were cleared efficiently. Quantitation by enzymatic assays revealed that the rates of cell loss and establishment differed for the two transformants. Ras-transformed cells exhibited a spindled-shaped morphology within the subcutis. In contrast, sis-transformed cells established a dichotomous morphology, with one subpopulation spreading modestly and another remaining rounded. 3T3 cells which established transiently became well-spread in a manner reminiscent of cells in culture. In conclusion, ras and sis oncogenes aid the parental 3T3 cells to overcome the clearance mechanisms at the subcutaneous injection site and modify 3T3 cell morphology as visualized in situ.
Journal Cover

September 1994
Volume 1 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
OCONNOR, K., & CULP, L. (1994). TOPOLOGICAL AND QUANTITATIVE-ANALYSES OF EARLY EVENTS IN TUMOR-FORMATION USING HISTOCHEMICALLY-TAGGED TRANSFORMED 3T3 CELLS. Oncology Reports, 1(5), 869-876.
MLA
OCONNOR, and LA CULP. "TOPOLOGICAL AND QUANTITATIVE-ANALYSES OF EARLY EVENTS IN TUMOR-FORMATION USING HISTOCHEMICALLY-TAGGED TRANSFORMED 3T3 CELLS." Oncology Reports Oncology Reports 1.5 (1994): 869-876.
Chicago
OCONNOR, and LA CULP. "TOPOLOGICAL AND QUANTITATIVE-ANALYSES OF EARLY EVENTS IN TUMOR-FORMATION USING HISTOCHEMICALLY-TAGGED TRANSFORMED 3T3 CELLS." Oncology Reports Oncology Reports 1 no. 5 (1994): 869-876.