Effect of Nma on growth inhibition by TGF-βa in human gastric carcinoma cell lines

  • Authors:
    • Takamitsu Sasaki
    • Tomonori Sasahira
    • Hideo Shimura
    • Seiyo Ikeda
    • Hiroki Kuniyasu
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/or.11.6.1219
  • Pages: 1219-1223
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Abstract

Non-metastatic gene A (nma) has a homologue DNA sequence to a gene of bone morphogenetic proteins and activin membrane-bound inhibitor (BAMBI), which negatively regulates TGF-β signaling. In this study, we analyzed the functional homology between Nma and BAMBI in human gastric carcinoma cell lines. Various levels of nma mRNA expression were detected by the RT-PCR technique in all human gastric carcinoma cell lines. Then, Nma antisense and sense S-oligodeoxynucleotide (ODN) were used to analyze the response of TGF-β to cell growth and invasion gastric carcinoma cell lines. The cell growth was inhibited by TGF-β in Nma antisense S-ODN treatment gastric carcinoma cell lines, MKN28, MKN1, MKN74 and TMK1. TGF-β reduced cell growth and invasive activity of MKN28 treated with Nma antisense S-ODN in a dose and time-dependent manner. Furthermore, lysates of Nma sense or antisense S-ODN treated MKN28 cells were immunoprecipitated with anti-TGFβR-I or anti-TGFβR-II antibody. The 29 kDa signal considered as Nma appeared in sense S-ODN treated MKN28 cells immunoprecipitated with anti-TGFβR-I. These results indicate that Nma negatively regulates TGF-β signaling, consequently playing an important role as one of the escape mechanisms from TGF-β-mediated growth control similarly to BAMBI, and induce cell growth and invasion in human gastric carcinoma cell lines.

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June 2004
Volume 11 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sasaki T, Sasahira T, Shimura H, Ikeda S and Kuniyasu H: Effect of Nma on growth inhibition by TGF-βa in human gastric carcinoma cell lines. Oncol Rep 11: 1219-1223, 2004
APA
Sasaki, T., Sasahira, T., Shimura, H., Ikeda, S., & Kuniyasu, H. (2004). Effect of Nma on growth inhibition by TGF-βa in human gastric carcinoma cell lines. Oncology Reports, 11, 1219-1223. https://doi.org/10.3892/or.11.6.1219
MLA
Sasaki, T., Sasahira, T., Shimura, H., Ikeda, S., Kuniyasu, H."Effect of Nma on growth inhibition by TGF-βa in human gastric carcinoma cell lines". Oncology Reports 11.6 (2004): 1219-1223.
Chicago
Sasaki, T., Sasahira, T., Shimura, H., Ikeda, S., Kuniyasu, H."Effect of Nma on growth inhibition by TGF-βa in human gastric carcinoma cell lines". Oncology Reports 11, no. 6 (2004): 1219-1223. https://doi.org/10.3892/or.11.6.1219