Methylation of multiple genes in prostate cancer and the relationship with clinicopathological features of disease

  • Authors: Rakesh Singal, Larry Ferdinand, Isildinha M. Reis, James J. Schlesselman
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  • Published online on: Wednesday, September 1, 2004
  • Pages: 631-637
  • DOI: 10.3892/or.12.3.631

Abstract

Promoter methylation plays an important role in the inactivation of tumor suppressor genes during tumorigenesis. We examined the methylation status of glutathione s-transferase Pi1 (GSTP1), retinoic acid receptor beta (RARB), CD44, E-cadherin (ECAD), RAS association domain family protein 1A (RASSF1A) and endothelin B receptor (EDNRB) genes in 81 prostate cancer and 42 benign prostatic hyperpasia specimens. Genomic DNA was isolated from archived formaldehyde-fixed and paraffin-embedded tissue blocks. Methylation-specific PCR (MSP) was carried out after bisulfite treatment of genomic DNA. Methylation frequencies in prostate cancer and benign prostatic hyperplasia were 72% and 5% for GSTP1, 40% and 0% for RARB, 72% and 38% for CD44, 61% and 14% for ECAD, 49% and 19% for RASSF1A and 72% and 62% for EDNRB, respectively. Methylation of GSTP1, RARB, CD44, ECAD and RASSF1A, but not of EDNRB was detected at a statistically higher frequency in prostate cancer than in the benign prostatic hypertrophy specimens. Methylation of RARB occurred more frequently in early onset (age <55 years) as compared to late onset disease (age >70 years) (odds ratio, 8.6; 95% CI, 1.4-51.4; P=0.02). Methylation of RARB also occurred more frequently in stage III as compared to stage II disease (odds ratio, 3.2; 95% CI, 1.1-8.8; P=0.03). A methylation index (MI) was calculated as the total number of genes methylated, excluding EDNRB. A trend toward higher MI was noted in stage III as compared to stage II disease, and in Gleason score 7 as compared to Gleason score 6 tumors. Our results suggest that the methylation of selected genes in prostate cancers correlates with clinicopathological features of poor prognosis.
Journal Cover

September 2004
Volume 12 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Singal, R., Ferdinand, L., Reis, I., & Schlesselman, J. (2004). Methylation of multiple genes in prostate cancer and the relationship with clinicopathological features of disease. Oncology Reports, 12(3), 631-637.
MLA
Singal, Ferdinand, Reis, and James Schlesselman. "Methylation of multiple genes in prostate cancer and the relationship with clinicopathological features of disease." Oncology Reports Oncology Reports 12.3 (2004): 631-637.
Chicago
Singal, Ferdinand, Reis, and James Schlesselman. "Methylation of multiple genes in prostate cancer and the relationship with clinicopathological features of disease." Oncology Reports Oncology Reports 12 no. 3 (2004): 631-637.