Inhibitory effect of ailanthoidol on 12-O-tetradecanoyl-phorbol-13-acetate-induced tumor promotion in mouse skin

  • Authors:
    • Yean-Jang Lee
    • Erl-Shyh Kao
    • Chia-Yih Chu
    • Wea-Lung Lin
    • Yea-Huey Chiou
    • Tsui-Hwa Tseng
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/or.16.4.921
  • Pages: 921-927
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Abstract

Many components derived from dietary or medicinal plants showing antioxidant and anti-inflammatory potential have been found to possess chemopreventive properties. In our previous study, we achieved the total synthesis of ailanthoidol (AT), a neolignan from Zanthoxylum ailanthoides or Salvia miltiorrhiza Bunge, which are used in Chinese traditional herbal medicine. In the present study, preliminarily, AT exhibited a radical quenching property by DPPH assay. Following this, we assessed the effect of AT on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative stress and inflammation in female CD-1 mouse skin which was closely linked to tumor promotion. The topical application of AT (0.5-2.5 mM; 200 µl) reduced the formation of hydrogen peroxide and inhibited the myeloperoxidase (MPO) activity in the mouse skin when compared with that of the TPA-treated alone group. In addition, AT presented a suppression effect on the TPA-induced hyperplasia and leukocyte infiltration in the epidermis and edema of mouse ears. Furthermore, it showed that AT inhibited the TPA-induced expression of COX-2 protein and ornithine decarboxylase (ODC) activity in epidermis. Finally, AT was evaluated for its ability to inhibit the TPA-induced promotion in skin tumors of female CD-1 mice. Topical application of AT 5 min prior to TPA (5 nmol) three times weekly for 12 weeks to mice which were initiated with benzo[a]pyrene (B[a]P) inhibited the incidence of skin tumors in mice and the average number of tumors per mice as compared to TPA-treated alone. These results indicate that AT possesses potential as a chemopreventive agent against tumor promotion.

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October 2006
Volume 16 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee Y, Kao E, Chu C, Lin W, Chiou Y and Tseng T: Inhibitory effect of ailanthoidol on 12-O-tetradecanoyl-phorbol-13-acetate-induced tumor promotion in mouse skin. Oncol Rep 16: 921-927, 2006
APA
Lee, Y., Kao, E., Chu, C., Lin, W., Chiou, Y., & Tseng, T. (2006). Inhibitory effect of ailanthoidol on 12-O-tetradecanoyl-phorbol-13-acetate-induced tumor promotion in mouse skin. Oncology Reports, 16, 921-927. https://doi.org/10.3892/or.16.4.921
MLA
Lee, Y., Kao, E., Chu, C., Lin, W., Chiou, Y., Tseng, T."Inhibitory effect of ailanthoidol on 12-O-tetradecanoyl-phorbol-13-acetate-induced tumor promotion in mouse skin". Oncology Reports 16.4 (2006): 921-927.
Chicago
Lee, Y., Kao, E., Chu, C., Lin, W., Chiou, Y., Tseng, T."Inhibitory effect of ailanthoidol on 12-O-tetradecanoyl-phorbol-13-acetate-induced tumor promotion in mouse skin". Oncology Reports 16, no. 4 (2006): 921-927. https://doi.org/10.3892/or.16.4.921