Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases

  • Authors:
    • Isabella Castellano
    • Anna Sapino
    • Riccardo Arisio
    • Giuseppe Viale
    • Gianni Bussolati
    • Roberto Bandelloni
    • Gaetano Barresi
    • Alessandra Bersiga
    • Cesare Bordi
    • Gerardo Botti
    • Fabia Cosimi
    • Emanuele D'Amore
    • Claudio Doglioni
    • Antonio Marchetti
    • Oscar Nappi
    • Francesco Romeo
    • Massimo Roncalli
    • Rosa Russo
    • Alfredo Santinelli
    • Luigi Giusto Spagnoli
    • Francesco Tanda
    • Paolo Tricomi
    • Gianpaolo Trentini
    • Fabrizio Zanconati
    • Monica Iurlaro
  • View Affiliations

  • Published online on: Thursday, May 1, 2008
  • Pages: 1271-1275
  • DOI: 10.3892/or.19.5.1271

Abstract

The aim of the present study was to evaluate the effectiveness of fluorescence in situ hybridisation (FISH), as a screening test, in moderately- (G2) or poorly- (G3) differentiated breast cancers of the ductal (IDC) and lobular (ILC) histotypes and distant metastases. HER2 FISH was performed on 486 G2 and 477 G3 both of IDC and ILC histotypes and in 241 metastases. A significant difference in the HER2 amplification was observed between G2 (14.8%) and G3 (31.9%), with no difference according to the histotype. However, the rate of amplification increased to 36% in the G2/hormone receptor-negative cases as compared to 10.6% in the G2/receptor-positive cases (p<0.0001). HER2 was amplified in 17% of metastases with some differences depending on the location. These data suggest that the HER2 FISH analysis may be an effective screening test in breast cancer metastases and G3 tumors, irrespective of the hormone receptor status or presence of lymphovascular invasion.
Journal Cover

May 2008
Volume 19 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Castellano, I., Sapino, A., Arisio, R., Viale, G., Bussolati, G., Bandelloni, R., Barresi, G., Bersiga, A., Bordi, C., Botti, G., Cosimi, F., D'Amore, E., Doglioni, C., Marchetti, A., Nappi, O., Romeo, F., Roncalli, M., Russo, R., Santinelli, A., Spagnoli, L., Tanda, F., Tricomi, P., Trentini, G., Zanconati, F., & Iurlaro, M. (2008). Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases. Oncology Reports, 19(5), 1271-1275.
MLA
Castellano, Sapino, Arisio, Viale, Bussolati, Bandelloni, Barresi, Bersiga, Bordi, Botti, Cosimi, D'Amore, Doglioni, Marchetti, Nappi, Romeo, Roncalli, Russo, Santinelli, Spagnoli, Tanda, Tricomi, Trentini, Zanconati, and Monica Iurlaro. "Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases." Oncology Reports Oncology Reports 19.5 (2008): 1271-1275.
Chicago
Castellano, Sapino, Arisio, Viale, Bussolati, Bandelloni, Barresi, Bersiga, Bordi, Botti, Cosimi, D'Amore, Doglioni, Marchetti, Nappi, Romeo, Roncalli, Russo, Santinelli, Spagnoli, Tanda, Tricomi, Trentini, Zanconati, and Monica Iurlaro. "Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases." Oncology Reports Oncology Reports 19 no. 5 (2008): 1271-1275.