Expression of E-selectin ligand-1 (CFR/ESL-1) on hepatic stellate cells: Implications for leukocyte extravasation and liver metastasis

  • Authors:
    • Marianne Antoine
    • Carmen G. Tag
    • Axel M. Gressner
    • Claus Hellerbrand
    • Paul Kiefer
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/or_00000230
  • Pages: 357-362
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Abstract

Leukocytes and tumor cells use E-selectin binding ligands to attach to activated endothelial cells expressing E-selectin during inflammation or metastasis. The cysteine-rich fibroblast growth factor receptor (CFR) represents the main E-selectin ligand (ESL-1) on granulocytes and its expression is exclusively modified by α(1,3)-fucosyltransferases IV or VII (FucT4 and FucT7). Hepatic stellate cells (HSC) are pericytes of liver sinusoidal endothelial cells. The activation of HSC and transdifferentiation into a myofibroblastic phenotype is involved in the repair of liver tissue injury, liver regeneration and angiogenesis of liver metastases. In the present study, we demonstrated that HSC expressed CFR together with FucT7 and exhibited a functional E-selectin binding activity on their cell surface. Since HSC appear to be oxygen-sensing cells, the expression of E-selectin binding activity was analyzed in HSC under a hypoxic atmosphere. While the expression of the glycoprotein CFR was unaffected by hypoxia, the cell-associated E-selectin binding activity decreased. However, under the same conditions, mRNA expression of the modifying enzyme FucT7 increased. The loss of E-selectin binding activity, therefore, appears to be neither the result of a reduced expression of the modifying transferase nor the expression of the backbone glycoprotein. After the transient transfection of HSC with CFR cDNA, the E-selectin binding activity (ESL-1) was efficiently released into the supernatant. Therefore, we hypothesize that under hypoxia, ESL-1 is shed from activated HSC. Our findings provide a novel perspective on the function of HSC in liver metastasis and inflammatory liver diseases.

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February 2009
Volume 21 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Antoine M, Tag CG, Gressner AM, Hellerbrand C and Kiefer P: Expression of E-selectin ligand-1 (CFR/ESL-1) on hepatic stellate cells: Implications for leukocyte extravasation and liver metastasis. Oncol Rep 21: 357-362, 2009
APA
Antoine, M., Tag, C.G., Gressner, A.M., Hellerbrand, C., & Kiefer, P. (2009). Expression of E-selectin ligand-1 (CFR/ESL-1) on hepatic stellate cells: Implications for leukocyte extravasation and liver metastasis. Oncology Reports, 21, 357-362. https://doi.org/10.3892/or_00000230
MLA
Antoine, M., Tag, C. G., Gressner, A. M., Hellerbrand, C., Kiefer, P."Expression of E-selectin ligand-1 (CFR/ESL-1) on hepatic stellate cells: Implications for leukocyte extravasation and liver metastasis". Oncology Reports 21.2 (2009): 357-362.
Chicago
Antoine, M., Tag, C. G., Gressner, A. M., Hellerbrand, C., Kiefer, P."Expression of E-selectin ligand-1 (CFR/ESL-1) on hepatic stellate cells: Implications for leukocyte extravasation and liver metastasis". Oncology Reports 21, no. 2 (2009): 357-362. https://doi.org/10.3892/or_00000230