The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells

  • Authors:
    • Hao Hu
    • Hui Ying Han
    • Yu Liang Wang
    • Xiao Peng Zhang
    • Chee Wai Chua
    • Y. C. Wong
    • Xiao Feng Wang
    • Ming Tat Ling
    • Ke Xin Xu
  • Corresponding author:
  • View Affiliations

  • Published online on: Wednesday, April 1, 2009
  • Pages: 1053-1059 DOI: 10.3892/or_00000323

Abstract

Recurrence and progression are the major problems in the treatment of bladder cancer. Increased expression of Id-1, a basic helix-loop-helix transcription factor, has recently been shown in several types of advanced cancer. Some studies have provided evidence to suggest that Id-1 can be considered a potential therapeutic target. The objective of this study was to investigate the role of Id-1 in the chemosensitivity of bladder cancer cells, and the effect of Id-1 on chemotherapeutic drug-induced apoptosis in bladder cancer cells. We compared the different sensitivity to epirubicin in RT112 and MGH-U1 cell lines with different Id-1 expression. Then, we transfected different vectors into RT112 and MGH-U1 respectively, and generated the stable Id-1 up-regulation and down-regulation transfectants. The results of cell viability assay showed up-regulation of Id-1 in RT112 leading to increased sensitivity in response to epirubicin, and down-regulation of Id-1 increased cellular sensitivity to epirubicin. Furthermore, the analysis of apoptosis related protein revealed that up-regulation of Id-1 suppressed epirubicin-induced apoptosis and down-regulation of Id-1 leading to increased epirubicin-induced apoptosis. Wound closure assay showed up-regulation of Id-1 leading to improved migration abilities of bladder cancer cells under chemotherapy. Our results suggest that up-regulation of Id-1 in bladder cancer cells lead to increased cell viability in response to epirubicin by its improved anti-apoptotic role, and down-regulation of Id-1 increases cellular sensitivity to epirubicin by increased anticancer drug-induced apoptosis.
Journal Cover

April 2009
Volume 21 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
2014 I.F. (Expected) ≥ 2.504 Ranked #33/202 Oncology
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APA
Hu, H., Han, H.Y., Wang, Y.L., Zhang, X.P., Chua, C.W., Wong, Y.C. ... Xu, K.X. (2009). The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells. Oncology Reports, 21, 1053-1059. http://dx.doi.org/10.3892/or_00000323
MLA
Hu, H., Han, H. Y., Wang, Y. L., Zhang, X. P., Chua, C. W., Wong, Y. C., Wang, X. F., Ling, M. T., Xu, K. X."The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells". Oncology Reports 21.4 (2009): 1053-1059.
Chicago
Hu, H., Han, H. Y., Wang, Y. L., Zhang, X. P., Chua, C. W., Wong, Y. C., Wang, X. F., Ling, M. T., Xu, K. X."The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells". Oncology Reports 21, no. 4 (2009): 1053-1059. http://dx.doi.org/10.3892/or_00000323