The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells

  • Authors: Hao Hu, Hui Ying Han, Yu Liang Wang, Xiao Peng Zhang, Chee Wai Chua, Y. C. Wong, Xiao Feng Wang, Ming Tat Ling, Ke Xin Xu
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  • Published online on: Wednesday, April 1, 2009
  • Pages: 1053-1059
  • DOI: 10.3892/or_00000323

Abstract

Recurrence and progression are the major problems in the treatment of bladder cancer. Increased expression of Id-1, a basic helix-loop-helix transcription factor, has recently been shown in several types of advanced cancer. Some studies have provided evidence to suggest that Id-1 can be considered a potential therapeutic target. The objective of this study was to investigate the role of Id-1 in the chemosensitivity of bladder cancer cells, and the effect of Id-1 on chemotherapeutic drug-induced apoptosis in bladder cancer cells. We compared the different sensitivity to epirubicin in RT112 and MGH-U1 cell lines with different Id-1 expression. Then, we transfected different vectors into RT112 and MGH-U1 respectively, and generated the stable Id-1 up-regulation and down-regulation transfectants. The results of cell viability assay showed up-regulation of Id-1 in RT112 leading to increased sensitivity in response to epirubicin, and down-regulation of Id-1 increased cellular sensitivity to epirubicin. Furthermore, the analysis of apoptosis related protein revealed that up-regulation of Id-1 suppressed epirubicin-induced apoptosis and down-regulation of Id-1 leading to increased epirubicin-induced apoptosis. Wound closure assay showed up-regulation of Id-1 leading to improved migration abilities of bladder cancer cells under chemotherapy. Our results suggest that up-regulation of Id-1 in bladder cancer cells lead to increased cell viability in response to epirubicin by its improved anti-apoptotic role, and down-regulation of Id-1 increases cellular sensitivity to epirubicin by increased anticancer drug-induced apoptosis.
Journal Cover

April 2009
Volume 21 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Hu, H., Han, H., Wang, Y., Zhang, X., Chua, C., Wong, Y., Wang, X., Ling, M., & Xu, K. (2009). The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells. Oncology Reports, 21(4), 1053-1059.
MLA
Hu, Han, Wang, Zhang, Chua, Wong, Wang, Ling, and Ke Xu. "The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells." Oncology Reports Oncology Reports 21.4 (2009): 1053-1059.
Chicago
Hu, Han, Wang, Zhang, Chua, Wong, Wang, Ling, and Ke Xu. "The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells." Oncology Reports Oncology Reports 21 no. 4 (2009): 1053-1059.