Crosstalk between adenosine receptor (A2A isoform) and ERα mediates ethanol action in MCF-7 breast cancer cells

  • Authors:
    • Nicolas Etique
    • Isabelle Grillier-Vuissoz
    • Julie Lecomte
    • Stephane Flament
  • View Affiliations

  • Published online on: April 1, 2009     https://doi.org/10.3892/or_00000311
  • Pages: 977-981
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Abstract

Alcohol consumption increases the risk of breast cancer but the underlying mechanisms are not well understood. We have shown previously that ethanol activates ER signalling pathway in a cAMP/PKA-mediated ligand-independent manner. Since the activation of A2A adenosine receptor (A2AAR) by ethanol has been reported in other cell types, here we tested if cross-talk between this Gs-coupled receptor and ERα could be involved in ethanol effects in breast cancer cells. Our study shows that A2AAR is expressed and functional in the hormone-dependent breast cancer cell line MCF-7. Interestingly, activation of this receptor by the selective agonist CGS21680 stimulates the transcription of progesterone receptor, a well known estrogen target gene. CGS21680 also stimulates the pEREtkLuc reporter activity in transfected MCF-7 cells, an effect antagonized by the antiestrogen ICI182,780. Moreover, CGS21680 stimulates the proliferation of MCF-7 cells similarly to E2. Finally, the A2AAR antagonist MSX-3 inhibits the ethanol-induced activation of ERα signalling pathway. These results demonstrate cross-talk between A2AAR and ERα that is involved in ethanol action. This could open new perspectives for the therapy of estrogen-dependent breast cancer.

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April 2009
Volume 21 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Etique N, Grillier-Vuissoz I, Lecomte J and Flament S: Crosstalk between adenosine receptor (A2A isoform) and ERα mediates ethanol action in MCF-7 breast cancer cells. Oncol Rep 21: 977-981, 2009
APA
Etique, N., Grillier-Vuissoz, I., Lecomte, J., & Flament, S. (2009). Crosstalk between adenosine receptor (A2A isoform) and ERα mediates ethanol action in MCF-7 breast cancer cells. Oncology Reports, 21, 977-981. https://doi.org/10.3892/or_00000311
MLA
Etique, N., Grillier-Vuissoz, I., Lecomte, J., Flament, S."Crosstalk between adenosine receptor (A2A isoform) and ERα mediates ethanol action in MCF-7 breast cancer cells". Oncology Reports 21.4 (2009): 977-981.
Chicago
Etique, N., Grillier-Vuissoz, I., Lecomte, J., Flament, S."Crosstalk between adenosine receptor (A2A isoform) and ERα mediates ethanol action in MCF-7 breast cancer cells". Oncology Reports 21, no. 4 (2009): 977-981. https://doi.org/10.3892/or_00000311