Two-dimensional differential in-gel electrophoresis for identification of gastric cancer-specific protein markers

  • Authors:
    • Cheng Wu
    • Zhiwen Luo
    • Xueyun Chen
    • Chaoqun Wu
    • Dingkang Yao
    • Peng Zhao
    • Lijie Liu
    • Bin Shi
    • Liang Zhu
  • View Affiliations

  • Published online on: June 1, 2009     https://doi.org/10.3892/or_00000371
  • Pages: 1429-1437
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Abstract

Gastric cancer is the second most common fatal malignancy in the world. Proteomics studies of clinical tumor samples have led to the identification of specific protein markers of gastric cancer detection and better understanding the carcinogenesis of gastric cancer. Gastric cancer tissue of epithelial origin and adjacent normal mucosa were examined in pair by fluorescence 2-D differential in-gel electrophoresis proteomics analysis utilizing 2-D PAGE protein separation. Intensity changes of 33 spots were detected with statistical significance. Twenty-two out of the 33 spots were identified by MALDI-TOF MS or MS/MS. Of the 9 up-regulated proteins, 7 were identified, including heat shock protein 60 (HSP60), mutant desmin, effector cell proteinase receptor 1 splice form 1b, hypothetical protein, unnamed protein product, and manganese superoxide dismutase (MnSOD), a protein similar to α-actin. Of the 20 down-regulated proteins, 16 were identified, including selenium binding protein 1, fibrinogen γ, HSP27, tubulin α 6, zinc finger protein 160, prostaglandin F synthase, and eukaryotic translation elongation factor 1 α 1. Our results suggest that MnSOD may be a potential serum marker for molecular diagnosis of gastric carcinoma, and DIGE is a useful technique for screening differentially expressed proteins in cancer tissues.

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June 2009
Volume 21 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu C, Luo Z, Chen X, Wu C, Yao D, Zhao P, Liu L, Shi B and Zhu L: Two-dimensional differential in-gel electrophoresis for identification of gastric cancer-specific protein markers. Oncol Rep 21: 1429-1437, 2009
APA
Wu, C., Luo, Z., Chen, X., Wu, C., Yao, D., Zhao, P. ... Zhu, L. (2009). Two-dimensional differential in-gel electrophoresis for identification of gastric cancer-specific protein markers. Oncology Reports, 21, 1429-1437. https://doi.org/10.3892/or_00000371
MLA
Wu, C., Luo, Z., Chen, X., Wu, C., Yao, D., Zhao, P., Liu, L., Shi, B., Zhu, L."Two-dimensional differential in-gel electrophoresis for identification of gastric cancer-specific protein markers". Oncology Reports 21.6 (2009): 1429-1437.
Chicago
Wu, C., Luo, Z., Chen, X., Wu, C., Yao, D., Zhao, P., Liu, L., Shi, B., Zhu, L."Two-dimensional differential in-gel electrophoresis for identification of gastric cancer-specific protein markers". Oncology Reports 21, no. 6 (2009): 1429-1437. https://doi.org/10.3892/or_00000371