Three-dimensional alginate spheroid culture system of murine osteosarcoma

  • Authors:
    • Koji Akeda
    • Akinobu Nishimura
    • Haruhiko Satonaka
    • Ken Shintani
    • Katsuyuki Kusuzaki
    • Akihiko Matsumine
    • Yuichi Kasai
    • Koichi Masuda
    • Atsumasa Uchida
  • View Affiliations

  • Published online on: November 1, 2009     https://doi.org/10.3892/or_00000527
  • Pages: 997-1003
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Abstract

Osteosarcoma (OS) is the most common primary malignant tumor of the bone and often forms pulmonary metastases, which are the most important prognostic factor. For further elucidation of the mechanism underlying the progression and metastasis of human OS, a culture system mimicking the microenvironment of the tumor in vivo is needed. We report a novel three-dimensional (3D) alginate spheroid culture system of murine osteosarcoma. Two different metastatic clones, the parental Dunn and its derivative line LM8, which has a higher metastatic potential to the lungs, were encapsulated in alginate beads to develop the 3D culture system. The beads containing murine OS cells were also transplanted into mice to determine their metastatic potential in vivo. In this culture system, murine OS cells encapsulated in alginate beads were able to grow in a 3D structure with cells detaching from the alginate environment. The number of detaching cells was higher in the LM8 cell line than the Dunn cell line. In the in vivo alginate bead transplantation model, the rate of pulmonary metastasis was higher with LM8 cells compared with that of Dunn cells. The cell characteristics and kinetics in this culture system closely reflect the original malignant potential of the cells in vivo.

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November 2009
Volume 22 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Akeda K, Nishimura A, Satonaka H, Shintani K, Kusuzaki K, Matsumine A, Kasai Y, Masuda K and Uchida A: Three-dimensional alginate spheroid culture system of murine osteosarcoma. Oncol Rep 22: 997-1003, 2009
APA
Akeda, K., Nishimura, A., Satonaka, H., Shintani, K., Kusuzaki, K., Matsumine, A. ... Uchida, A. (2009). Three-dimensional alginate spheroid culture system of murine osteosarcoma. Oncology Reports, 22, 997-1003. https://doi.org/10.3892/or_00000527
MLA
Akeda, K., Nishimura, A., Satonaka, H., Shintani, K., Kusuzaki, K., Matsumine, A., Kasai, Y., Masuda, K., Uchida, A."Three-dimensional alginate spheroid culture system of murine osteosarcoma". Oncology Reports 22.5 (2009): 997-1003.
Chicago
Akeda, K., Nishimura, A., Satonaka, H., Shintani, K., Kusuzaki, K., Matsumine, A., Kasai, Y., Masuda, K., Uchida, A."Three-dimensional alginate spheroid culture system of murine osteosarcoma". Oncology Reports 22, no. 5 (2009): 997-1003. https://doi.org/10.3892/or_00000527