14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma

  • Authors:
    • Sylvia Yat-Yee Chan
    • Ka-Fai To
    • Sing-Fai Leung
    • William Wai-Lun Yip
    • Michael Ko-Fung Mak
    • Grace Tin-Yun Chung
    • Kwok-Wai Lo
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/or_00000941
  • Pages: 949-955
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Abstract

In this study, we investigated the expression of 14-3-3σ tumor suppressor gene in a panel of NPC cell lines, xenografts and primary tumors. Our objective was to determine the correlation between 14-3-3σ expression and clinical outcome in NPC. We detected reduced 14-3-3σ expression in 5/6 NPC tumor lines by quantitative RT-PCR and Western blotting. By immunohistochemical staining, significant down-regulation of 14-3-3σ was also found in 26/72 (36.1%) primary tumors of NPC patients, who were treated with curative radiotherapy. Promoter methylation was confirmed in a subset of primary tumors by methylation-specific PCR analysis. Importantly, we demonstrated that 14-3-3σ expression is significantly associated with both overall survival (OS) and cancer-specific survival (CSS), but not with the clinical staging of NPC patients. The low 14-3-3σ expression was associated with improved overall (p=0.029) and cancer-specific survival (p=0.042) on univariate analysis. 14-3-3σ expression and staging were also independent variables to all the prognostic factors under multivariate analysis. In conclusion, low expression of 14-3-3σ appears to be a valuable marker for better survival in patient with NPC. These results provide the evidence that 14-3-3σ expression is a significant prognostic factor for NPC patients.

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October 2010
Volume 24 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chan SY, To K, Leung S, Yip WW, Mak MK, Chung GT and Lo K: 14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma . Oncol Rep 24: 949-955, 2010
APA
Chan, S.Y., To, K., Leung, S., Yip, W.W., Mak, M.K., Chung, G.T., & Lo, K. (2010). 14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma . Oncology Reports, 24, 949-955. https://doi.org/10.3892/or_00000941
MLA
Chan, S. Y., To, K., Leung, S., Yip, W. W., Mak, M. K., Chung, G. T., Lo, K."14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma ". Oncology Reports 24.4 (2010): 949-955.
Chicago
Chan, S. Y., To, K., Leung, S., Yip, W. W., Mak, M. K., Chung, G. T., Lo, K."14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma ". Oncology Reports 24, no. 4 (2010): 949-955. https://doi.org/10.3892/or_00000941