3,3'-Diindolylmethane inhibits migration and invasion of human cancer cells through combined suppression of ERK and AKT pathways

  • Authors: Shilpi Rajoria, Robert Suriano, Yushan Lisa Wilson, Stimson P. Schantz, Augustine Moscatello, Jan Geliebter, Raj K. Tiwari
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  • Published online on: Tuesday, December 7, 2010
  • Pages: 491-497
  • DOI: 10.3892/or.2010.1076

Abstract

Metastasis of cancer is a multifactorial disease and is the main cause of death in patients with malignancy. This disease demands treatments which may target multiple dysregulated cellular pathways in cancer cells. The anti-tumor and anti-metastatic properties of natural products in cancer prevention have been confirmed by several epidemiological studies, with cruciferous vegetables being especially protective against many cancers. In this study, we evaluated the anti-carcinogenic effects of 3,3'-diindolylmethane (DIM), which is a bioactive compound present in cruciferous vegetables and a widely used dietary supplement, on events commonly observed during metastasis using in vitro adhesion, migration and invasion assays. Our results indicate that DIM inhibits human cancer cell in vitro proliferation, adhesion, migration and invasion. Western blot analyses show that this inhibition of cell proliferation by DIM is exerted by combined suppression of AKT and ERK pathways. Furthermore, DIM also leads to down-regulation of G1-S cell cycle markers: cyclin D1, cdk6 and cdk4. These findings may lead to development of a novel preventive and/or therapeutic dietary supplement for patients diagnosed with cancer or predisposed to developing certain cancers.
Journal Cover

February 2011
Volume 25 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Rajoria, S., Suriano, R., Wilson, Y., Schantz, S., Moscatello, A., Geliebter, J., & Tiwari, R. (2011). 3,3'-Diindolylmethane inhibits migration and invasion of human cancer cells through combined suppression of ERK and AKT pathways. Oncology Reports, 25(2), 491-497.
MLA
Rajoria, Suriano, Wilson, Schantz, Moscatello, Geliebter, and Raj Tiwari. "3,3'-Diindolylmethane inhibits migration and invasion of human cancer cells through combined suppression of ERK and AKT pathways." Oncology Reports Oncology Reports 25.2 (2011): 491-497.
Chicago
Rajoria, Suriano, Wilson, Schantz, Moscatello, Geliebter, and Raj Tiwari. "3,3'-Diindolylmethane inhibits migration and invasion of human cancer cells through combined suppression of ERK and AKT pathways." Oncology Reports Oncology Reports 25 no. 2 (2011): 491-497.