Effect of triptolide on focal adhesion kinase and survival in MCF-7 breast cancer cells

  • Authors:
    • Bee-Jen Tan
    • Bee-Huey Tan
    • Gigi N.C. Chiu
  • View Affiliations

  • Published online on: July 28, 2011     https://doi.org/10.3892/or.2011.1406
  • Pages: 1315-1321
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Abstract

Triptolide, a diterpene from Tripterygium wilfordii, has been shown to have potent anticancer activity, exerting its effects through multiple molecular targets and signaling pathways. Yet, its effect on focal adhesion kinase (FAK), a non-receptor tyrosine kinase overexpressed in breast cancer that regulates cellular adhesion and survival, has not been reported. The current study is the first to report on the effect of triptolide on FAK expression, cell adhesion and survival using MCF-7 breast cancer cells. Triptolide significantly reduced MCF-7 anchorage-independent growth in a concentration-dependent manner. Cell rounding and detachment from culture plates were observed as early as 8 h, with significant cell detachment observed after 24 h of triptolide treatment. The adhesion potential of triptolide-treated MCF-7 cells to Matrigel was also compromised. Triptolide induced concentration- and time-dependent cleavage of FAK and PARP, which was dependent on caspase activation. The pan-caspase inhibitor, zVAD-fmk, was the only inhibitor that could significantly reduce FAK and PARP cleavage and cell detachment. However, the presence of zVAD-fmk failed to significantly reverse triptolide-induced cell death. Finally, triptolide-induced FAK cleavage was specific to MCF-7 cells, as no cleaved FAK was observed in MDA-MB-231 cells. In conclusion, our data present the first evidence of triptolide-mediated induction of FAK cleavage that correlates with cell detachment and loss of adhesion potential to the extracellular matrix.

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November 2011
Volume 26 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Tan B, Tan B and Chiu GN: Effect of triptolide on focal adhesion kinase and survival in MCF-7 breast cancer cells. Oncol Rep 26: 1315-1321, 2011
APA
Tan, B., Tan, B., & Chiu, G.N. (2011). Effect of triptolide on focal adhesion kinase and survival in MCF-7 breast cancer cells. Oncology Reports, 26, 1315-1321. https://doi.org/10.3892/or.2011.1406
MLA
Tan, B., Tan, B., Chiu, G. N."Effect of triptolide on focal adhesion kinase and survival in MCF-7 breast cancer cells". Oncology Reports 26.5 (2011): 1315-1321.
Chicago
Tan, B., Tan, B., Chiu, G. N."Effect of triptolide on focal adhesion kinase and survival in MCF-7 breast cancer cells". Oncology Reports 26, no. 5 (2011): 1315-1321. https://doi.org/10.3892/or.2011.1406