miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin

  • Authors:
    • Shao-Hua Lu
    • Xing-Jun Jiang
    • Ge-Lei Xiao
    • Ding-Yang Liu
    • Xian-Rui Yuan
  • View Affiliations

  • Published online on: August 29, 2014     https://doi.org/10.3892/or.2014.3455
  • Pages: 2104-2110
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Abstract

A number of microRNAs have been identified to be important regulators of tumorigenesis. Previous research has shown that miR-124 is abundantly expressed in normal brain tissue; however, only a few reports have focused on the biological impact of miR-124 on glioma cells, and the underlying mechanisms need to be elucidated. Therefore, we investigated the effect of miR-124a on glioma cell proliferation and invasion; furthermore, the underlying molecular mechanism was examined. The present study demonstrated that miR-124a expression was downregulated in human glioma tissues, and its expression level was negatively correlated with the pathological grade of the glioma. Restoration of miR-124a inhibited glioma cell proliferation and invasion in vitro. Furthermore, we found that miR-124a directly targeted and suppressed IQ motif containing GTPase activating protein 1 (IQGAP1), a well-known regulator of actin dynamics and cell motility. RNA interference assay showed that IQGAP1 knockdown led to downregulation of β-catenin and downstream cyclin D1. Taken together, our study revealed that miR-124a could inhibit glioma cell proliferation and invasion by blocking the expression of the IQGAP1 gene and downstream β-catenin and cyclin D1. This research may provide a useful molecular therapy for gliomas.
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November-2014
Volume 32 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Lu S, Jiang X, Xiao G, Liu D and Yuan X: miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin. Oncol Rep 32: 2104-2110, 2014
APA
Lu, S., Jiang, X., Xiao, G., Liu, D., & Yuan, X. (2014). miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin. Oncology Reports, 32, 2104-2110. https://doi.org/10.3892/or.2014.3455
MLA
Lu, S., Jiang, X., Xiao, G., Liu, D., Yuan, X."miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin". Oncology Reports 32.5 (2014): 2104-2110.
Chicago
Lu, S., Jiang, X., Xiao, G., Liu, D., Yuan, X."miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin". Oncology Reports 32, no. 5 (2014): 2104-2110. https://doi.org/10.3892/or.2014.3455