Open Access

Knockdown of glucose-regulated protein 78 enhances poly(ADP-ribose) polymerase cleavage in human pancreatic cancer cells exposed to endoplasmic reticulum stress

  • Authors:
    • Xia Jiang
    • Tatsuo Kanda
    • Shingo  Nakamoto
    • Yuki  Haga
    • Reina  Sasaki
    • Masato Nakamura
    • Shuang  Wu
    • Rintaro Mikata
    • Osamu  Yokosuka
  • View Affiliations

  • Published online on: October 6, 2014     https://doi.org/10.3892/or.2014.3533
  • Pages: 2343-2348
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study examined the expression of glucose‑regulated protein 78 (GRP78/Bip) in human pancreatic cancer cell lines and the effect of knockdown of GRP78 on the cleavage of poly(ADP-ribose) polymerase (PARP). Human pancreatic cancer cell lines (KP-2, MIAPaCa-2, Panc-1 and SUIT-2), constitutively expressed GRP78. We also demonstrated that ER stress induced by thapsigargin upregulated protein levels of GRP78. In the presence of thapsigargin, knockdown of GRP78 enhanced the PARP cleavage in the human pancreatic cancer cells. These results provide evidence that GRP78 is a potential therapeutic target for ‘difficult-to-treat’ pancreatic cancer, in which ER stress signaling in part falls into disorder.
View Figures
View References

Related Articles

Journal Cover

December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jiang X, Kanda T, Nakamoto S, Haga Y, Sasaki R, Nakamura M, Wu S, Mikata R and Yokosuka O: Knockdown of glucose-regulated protein 78 enhances poly(ADP-ribose) polymerase cleavage in human pancreatic cancer cells exposed to endoplasmic reticulum stress. Oncol Rep 32: 2343-2348, 2014
APA
Jiang, X., Kanda, T., Nakamoto, S., Haga, Y., Sasaki, R., Nakamura, M. ... Yokosuka, O. (2014). Knockdown of glucose-regulated protein 78 enhances poly(ADP-ribose) polymerase cleavage in human pancreatic cancer cells exposed to endoplasmic reticulum stress. Oncology Reports, 32, 2343-2348. https://doi.org/10.3892/or.2014.3533
MLA
Jiang, X., Kanda, T., Nakamoto, S., Haga, Y., Sasaki, R., Nakamura, M., Wu, S., Mikata, R., Yokosuka, O."Knockdown of glucose-regulated protein 78 enhances poly(ADP-ribose) polymerase cleavage in human pancreatic cancer cells exposed to endoplasmic reticulum stress". Oncology Reports 32.6 (2014): 2343-2348.
Chicago
Jiang, X., Kanda, T., Nakamoto, S., Haga, Y., Sasaki, R., Nakamura, M., Wu, S., Mikata, R., Yokosuka, O."Knockdown of glucose-regulated protein 78 enhances poly(ADP-ribose) polymerase cleavage in human pancreatic cancer cells exposed to endoplasmic reticulum stress". Oncology Reports 32, no. 6 (2014): 2343-2348. https://doi.org/10.3892/or.2014.3533