Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo

  • Authors:
    • Wei Meng
    • Pei-Song Wang
    • Jia Liu
    • Shuai Xue
    • Gui-Min Wang
    • Xian-Ying Meng
    • Guang Chen
  • View Affiliations

  • Published online on: September 16, 2014     https://doi.org/10.3892/or.2014.3483
  • Pages: 2411-2420
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Abstract

NIN1/RPN12 binding protein 1 homolog (NOB1), a ribosome assembly factor, plays critical roles in tumor progression and development. Previously, we reported that overexpression of NOB1 is correlated with the prognosis of patients with papillary thyroid carcinoma (PTC). Little is known, however, concerning its role in PTC. The aims of the present study were to investigate the association of NOB1 expression with tumor growth and radiosensitivity of human PTC. A recombinant adenovirus expression vector carrying NOB1 was constructed and then infected into the human PTC cell line TPC-1. Cell proliferation, cell cycle distribution, apoptosis, migration and invasion in vitro and tumor growth in vivo were determined after downregulation of NOB1 by RNAi. Additionally, the in vitro and in vivo radiosensitivity of PTC cells was determined by clonogenic cell survival assay and a mouse xenograft model, respectively. The results showed that downregulation of NOB1 expression using RNAi in TPC-1 cells significantly inhibited cell proliferation, migration and invasion and induced cell apoptosis in vitro, and suppressed tumor growth in vivo, as well as enhanced the in vitro and in vivo radiosensitivity of PTC cells. Moreover, our results also showed that downregulation of NOB1 was able to significantly activate constitutive phosphorylation of p38 MAPK, which might contribute to the inhibition of PTC cell growth. These findings suggest that NOB1 may be a potential therapeutic target for the treatment of PTC.
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December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Meng W, Wang P, Liu J, Xue S, Wang G, Meng X and Chen G: Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo. Oncol Rep 32: 2411-2420, 2014
APA
Meng, W., Wang, P., Liu, J., Xue, S., Wang, G., Meng, X., & Chen, G. (2014). Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo. Oncology Reports, 32, 2411-2420. https://doi.org/10.3892/or.2014.3483
MLA
Meng, W., Wang, P., Liu, J., Xue, S., Wang, G., Meng, X., Chen, G."Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo". Oncology Reports 32.6 (2014): 2411-2420.
Chicago
Meng, W., Wang, P., Liu, J., Xue, S., Wang, G., Meng, X., Chen, G."Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo". Oncology Reports 32, no. 6 (2014): 2411-2420. https://doi.org/10.3892/or.2014.3483