Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells

  • Authors:
    • Xiuyuan Ou
    • Yan Chen
    • Xinxin Cheng
    • Xumeng Zhang
    • Qiyang He
  • View Affiliations

  • Published online on: September 23, 2014     https://doi.org/10.3892/or.2014.3512
  • Pages: 2803-2809
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Resveratrol, a natural polyphenolic phytochemical, has received considerable attention due to its potential chemopreventive and chemotherapeutic properties. In the present study, we first evaluated the growth-inhibitory effect of resveratrol on HepG2 cells and explored the underlying molecular mechanisms. Resveratrol inhibited proliferation and induced apoptosis in HepG2 cells via activation of caspase-9 and caspase-3, upregulation of the Bax/Bcl-2 ratio and induction of p53 expression. Cell cycle analysis demonstrated that resveratrol arrested cell cycle progression in the G1 and S phase. We further focused on the combination of matrine, a natural component extracted from the traditional Chinese medical herb Sophora flavescens Ait., as a mechanism to potentiate the growth-inhibitory effect of resveratrol on HepG2 cells. Both MTT and colony formation assay results indicated that the combined treatment of resveratrol and matrine exhibited a synergistic antiproliferative effect. In addition, resveratrol-induced apoptosis was significantly enhanced by matrine, which could be attributed to activation of caspase-3 and caspase-9, downregulation of survivin, induction of reactive oxygen species (ROS) generation and disruption of mitochondria membrane potential (Δψm). Our findings suggest that the combination treatment of resveratrol and matrine is a promising novel anticancer strategy for liver cancer; it also provides new insights into the mechanisms of combined therapy.
View Figures
View References

Related Articles

Journal Cover

December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ou X, Chen Y, Cheng X, Zhang X and He Q: Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncol Rep 32: 2803-2809, 2014
APA
Ou, X., Chen, Y., Cheng, X., Zhang, X., & He, Q. (2014). Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncology Reports, 32, 2803-2809. https://doi.org/10.3892/or.2014.3512
MLA
Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q."Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells". Oncology Reports 32.6 (2014): 2803-2809.
Chicago
Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q."Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells". Oncology Reports 32, no. 6 (2014): 2803-2809. https://doi.org/10.3892/or.2014.3512