Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression

  • Authors:
    • Congfei Wang
    • Weiwei Zhang
    • Mingjuan Fu
    • Aiqin Yang
    • Heguang Huang
    • Jieming Xie
  • View Affiliations

  • Published online on: November 12, 2014     https://doi.org/10.3892/or.2014.3599
  • Pages: 383-390
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Abstract

A gemcitabine (GEM)-resistant human pancreatic cancer cell line (PANC-1RG7) was established in vitro by gradually increasing GEM concentrations and cloning cell cultures to develop a cellular model of acquired drug resistance studies. We found that PANC-1RG7 cells exhibited significantly different morphological characteristics from parental cells. PANC-1RG7 cells grew slowly (p<0.05), yet the cell cycle remained unchanged (p>0.05). PANC-1RG7, with a resistance index to GEM of 39.9, showed cross-resistance characteristics to methotrexate, gefitinib, cisplatin and 5-fluorouracil. The proliferation inhibition of GEM was significantly reduced in vivo (p<0.05). The known resistance-associated genes and proteins we detected remained unchanged, with the exception of cytidine deaminase, multidrug resistance-related protein and breast cancer resistance protein genes, which decreased; by contrast, 5'-nucleotidase, ribonucleotide reductase (RRM) 1 and RRM2 proteins increased (p<0.05). Therefore, a cell line with acquired GEM resistance was established successfully. Resistance was acquired by overexpressing RRM1 and RRM2 proteins.
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January-2015
Volume 33 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang C, Zhang W, Fu M, Yang A, Huang H and Xie J: Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression. Oncol Rep 33: 383-390, 2015
APA
Wang, C., Zhang, W., Fu, M., Yang, A., Huang, H., & Xie, J. (2015). Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression. Oncology Reports, 33, 383-390. https://doi.org/10.3892/or.2014.3599
MLA
Wang, C., Zhang, W., Fu, M., Yang, A., Huang, H., Xie, J."Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression". Oncology Reports 33.1 (2015): 383-390.
Chicago
Wang, C., Zhang, W., Fu, M., Yang, A., Huang, H., Xie, J."Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression". Oncology Reports 33, no. 1 (2015): 383-390. https://doi.org/10.3892/or.2014.3599